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Heart 1998;80:146-150; doi:10.1136/hrt.80.2.146
Copyright © 1998 BMJ Publishing Group Ltd & British Cardiovascular Society

Heart 1998;80:146-150 ( August )

S-Nitrosoglutathione inhibits platelet activation and deposition in coronary artery saphenous vein grafts in vitro and in vivo

E Salas,a E J Langford,b M T Marrinan,c J F Martin,d S Moncada,d A J de Belderb

a Department of Gynecology, Obstetrics and Pharmacology, University of Alberta, Edmonton, Canada, b Department of Cardiology, King's College Hospital, London, UK, c Department of Cardiothoracic Surgery, King's College Hospital, d Cruciform Project, University College, London, UK

Correspondence to: Dr A J de Belder, Department of Cardiology, King's College Hospital, Denmark Hill, London SE5 9RS, UK.

Accepted for publication 4 March 1998

Objective---To investigate platelet activation and deposition in human saphenous vein and internal mammary artery grafts following coronary artery bypass in vitro and in vivo, as well as inhibition of activation by the platelet selective nitric oxide donor S-nitrosoglutathione (GSNO).
Design---Controlled in vitro and in vivo studies.
Setting---Tertiary cardiac centre.
Patients---24 patients undergoing coronary artery bypass surgery requiring vein and artery grafts.
Interventions---In vitro: human platelet rich plasma was perfused through segments of vein and artery, with or without GSNO 10-6 M, and the platelet count was measured in the effluent. In vivo: indium-111 labelled antibody against the platelet alpha  granule protein GMP-140 was injected at the end of coronary bypass grafting and gamma  counts were compared between vein and artery grafts with or without systemic infusion of GSNO (40 nmol/min).
Results---In vitro: platelet count in perfused vein (< 70% of baseline) decreased more than in artery segments (89-94% of baseline) (p < 0.001). The platelet count was unchanged with GSNO in vein and artery segments. In vivo: gamma  counts were greater at all time points over vein than artery grafts (p < 0.05), and were reduced by infusion of GSNO (p < 0.05).
Conclusions---Platelet activation is greater in vein than in artery grafts in vitro and in vivo. Activation, which contributes to early vein graft failure, was inhibited by GSNO.

Keywords: coronary artery bypass surgery;  platelet activation;  S-nitrosoglutathione;  ischaemic heart disease

© 1998 by Heart
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