ABSTRACTS

Abstracts from The British Society for Cardiovascular Research Spring Meeting 2007

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001 DEVELOPMENT OF A MOUSE ISOLATED HEART MODEL OF ISCHAEMIA INDUCED VENTRICULAR FIBRILLATION

C. L. Stables, M. Avkiran, M. J. Curtis

Cardiovascular Division, King’s College London, St Thomas’ Hospital, London, UK

The first 150 words of the full text of this article appear below.

Functional genomics could be used to identify novel modulators of ischaemia induced lethal arrhythmias, but this application has been limited by the infrequency of ventricular fibrillation (VF) in the wildtype murine heart. The aim of this study was to develop a robust mouse isolated-heart model of ischaemia/reperfusion induced VF. The initial strategy was to reintroduce catecholamines and angiotensin II (A-II), putative arrhythmogens in vivo that are ordinarily absent in perfused heart preparations. Hearts from male T/O mice were Langendorff perfused with control solution (standard Krebs modified to contain 2.4 mM Ca²⁺ and 3 mM K⁺) before being perfused with a test solution containing catecholamines (noradrenaline 313 nM, adrenaline 75 nM) and/or A-II (100 pM), or control (n = 10 per group). Hearts were then subjected to 30 min regional ischaemia by ligation of the left main coronary artery, followed by reperfusion. The ECG was monitored throughout. Catecholamine perfusion significantly . . . [Full text of this article]

D. Ribé, J-M. Li

Cardiovascular Research Theme, SBMS, University of Surrey, Guildford, UK

S. Rahman¹, A. Lundkvist², C. Xu¹, R. Sumathipala¹, T. Fujisawa¹, E. Wijelath³, Y. Patel¹, G. Kanaganayagum¹, M. Sobel³, H. Gerhardt²

¹ Laboratory of Thrombosis and Vascular Remodelling, Division of Cardiovascular Medicine, King’s College London School of Medicine, London, UK
² Vascular Biology Laboratory, Cancer Research UK, London, UK
³ Department of Surgery, Division of Vascular Surgery, Veterans Affairs Puget Sound Health Care System and the University of Washington School of Medicine, Seattle, USA

S. Ahmad, M. Cudmore, B. Al-Ani, K. Chudasama, T. Fujisawa, P. Hewett, A. Ahmed

Department of Reproductive and Vascular Biology, Institute of Biomedical Research, University of Birmingham, UK

F. L. Moseley¹, M. E. Faircloth², W. Lockwood¹, M. S. Marber², K. A. Bicknell¹, G. Brooks¹

¹ School of Pharmacy, The University of Reading, UK
² The Rayne Institute, Kings College London, St Thomas’ Hospital, London, UK

K. A. Bicknell, I. Kavanagh, W. Lockwood, S. T. Lynch, G. Brooks

School of Pharmacy, University of Reading, UK

M. Movassagh, A. Philpott

University of Cambridge, Division of Cardiovascular Medicine, Centre for Clinical Investigation, Addenbrooke’s Hospital, Cambridge, UK

V. T. George, J-M. Li

Cardiovascular Research Theme, SBMS, University of Surrey, UK

H. Mattar¹, K. Zacharias², A. Afzal¹, N. Carter¹, A. Bakhai²

¹ Division of Clinical Developmental Sciences, St George’s University of London
² Barnet General Hospital, London, UK

B. Wright¹, J. A. Lovegrove², J. M. Gibbins¹

¹ Schools of Biological Sciences and
² Food Biosciences, University of Reading, UK

K. K. Deepak, K. Madan, A. Jaryal, A. Roy, N. Pandit, D. Prabhakaran, R. M. Pandey, S. K. Reddy

Departments of Physiology, Cardiology and Nuclear Medicine, All India Institute of Medical Sciences, New Delhi, India

A. K. Jaryal, R. Khadka, R. Narang, C. D. Patel, K. K. Deepak

Departments of Physiology, Cardiology, and Nuclear Medicine, All India Institute of Medical Sciences, New Delhi, India

J. Z. Yogaratnam, G. Laden, J. Greenman, A. M. Seymour, A. Cale, S. Griffin

Department of Biological Sciences, University of Hull, UK

G. Mckoy, K. A. Bicknell, K. Patel, G. Brooks

Schools of Pharmacy and Biological Sciences, University of Reading, UK

L. A. Moraes¹, D. Bishop-Bailey², J. M. Gibbins¹

¹ School of Biological Sciences, University of Reading, UK
² William Harvey Research Institute, Bart’s and the London, Queen Mary’s School of Medicine and Dentistry, London, UK

A. B. Gerry, D. S. Leake

School of Biological Sciences, University of Reading, UK

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