Increased granzyme B production from peripheral blood mononuclear cells in patients with acute coronary syndrome
R Tsuru, H Kondo, Y Hojo, M Gama, O Mizuno, T Katsuki, K Shimada, M Kikuchi, and T Yashiro
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Granzyme B expression in ruptured coronary plaques
Panel A: Figure shows the representative expression of granzyme B in ruptured coronary plaques obtained from a patient with unstable angina. Green fluorescence indicates granzyme B expression and blue fluorescence with 4,6-diamino-2-phenylindole (DAPI) demonstrates nuclear staining. Granular distribution of fluorescein isothiocyanate (FITC)-labeled granzyme B in mononuclear cells was observed.
Panel B: Positive control for granzyme B expression. Phorbol 12-myristate 13-acetate-stimulated peripheral blood mononuclear cells were stained with FITC-labeled anti-granzyme B antibody and DAPI.
Left panel: merged image stained with FITC-labeled granzyme B and DAPI. Right panel: the image stained with FITC-labeled granzyme B alone.
Yellow arrows indicate FITC-labeled granzyme B in the cells.
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