Aim To study the effect of monocytic extracellular vesicles (EVs) on proliferation of human vascular smooth muscle cells (VSMCs).
Methods EVs were isolated from culture medium supernatants of THP-1 cells by differential centrifugation. The protein concentration of EVs was measured using BCA assay. The proliferation of VSMCs was evaluated by [3H] thymidine incorporation and Cell Counting Kit-8 (CCK-8). The phosphorylation and expression of protein were detected by immunoblotting.
Results Monocytic EVs promoted the proliferation of VSMCs in a concentration- and time-dependent manner. EVs from monocytes pretreated with LPS for 1h exhibited significantly stronger effects on VSMCs proliferation. EVs stimulated the phosphorylation of ERK, JNK and P38, and the expressions of c-fos and c-jun in VSMCs.
Conclusion EVs from monocytes could promote the proliferation of VSMCs, which is associated with the activation of MAPK pathways.
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