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215 Increased Atherosclerotic Plaque Macrophage Content following Streptococcus Pneumoniae Pneumonia
  1. Rohit Bazaz1,
  2. Sheila Francis2,
  3. David Dockrell3
  1. 1University of Sheffield
  2. 2Department of Cardiovascular Science, University of Sheffield
  3. 3Department of Infection and Immunity

Abstract

Background Clinical studies consistently find an increase in the risk of acute coronary syndrome (ACS) within 1–2 weeks after a respiratory infection. It has yet to be established whether a causal relationship exists. Post mortem studies have revealed that the majority of ACS events occur as a result of atherosclerotic plaque disruption. We hypothesise that the systemic inflammatory response to pneumonia leads to acute localised inflammatory changes within established atherosclerotic plaques, favouring plaque instability, thereby resulting in ACS. Our objective was to investigate the effect of resolving pneumonia on atherosclerotic plaque composition.

Materials and methods 11–13 week old male ApoE-/- mice, a well-established model of atherosclerosis, were fed an atherogenic diet for 7–8 weeks before intranasal infection with 5 × 105 cfu type 4 Streptococcus pneumoniae or mock infection. All mice received 3 doses of 100 mg/kg subcutaneous amoxicillin, 12 hly, the first dose given at 24 h post infection. Mice were sacrificed 2 weeks post infection and the heart and brachiocephalic artery excised. Formalin fixed, paraffin embedded aortic sinus and brachiocephalic artery atherosclerotic plaque sections were analysed by histological and immunohistochemical staining.

Results This model of atherosclerotic plaque development following pneumonia resulted in high rates of bacteraemia at 24 h post infection and high survival rates at 5 days post infection (92% and 83% respectively). Streptococcus pneumoniae infection was associated with significantly increased aortic sinus atherosclerotic plaque macrophage content (18.1 vs. 8.0%; p < 0.05) (Figure 1). No significant differences in aortic sinus plaque burden, smooth muscle or collagen content were found following infection. Relatively few mice developed significant atherosclerosis at the level of the brachiocephalic artery and no significant differences were found between the two groups in plaques at this site, including buried cap formation.

Abstract 215 Figure 1

Representative paraffin embedded aortic sinus sections from an infected (Figure 1a) and mock infected (Figure 1b) ApoE–/– mouse showing increased immunostaining for MAC-3 antigen in the infected mouse sample. Scale bar represents 50 µm. P = plaque area

Discussion In this murine model, pneumococcal pneumonia resulted in increased atherosclerotic plaque macrophage content, a marker of plaque instability. Pneumonia may therefore lead to an increased propensity for atherosclerotic plaques to rupture, although further work is required to assess the polarisation of macrophages in atherosclerotic plaques post pneumonia.

  • Atherosclerosis
  • Plaque
  • Pneumonia

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