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7 Quantifying the bio-distribution of transplanted HESC-ECS in a murine model of HLI by QPCR
  1. Lucija Fleisinger1,
  2. Mark G MacAskill1,
  3. Joanne C Mountford2,
  4. Andrew H Baker1,
  5. David E Newby1,
  6. Patrick WF Hadoke1
  1. 1University/BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK
  2. 2Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK

Abstract

Introduction Beneficial effects of stem/progenitor cell therapies for the treatment of ischaemic diseases have been established; however, there remains conflicting evidence on whether cells act by incorporation into the existing vasculature or by paracrine effects.

Objective This study aims to quantify the bio-distribution of transplanted, proangiogenic, human embryonic stem cell-derived endothelial cells (hESC-ECs) in a murine model of hind limb ischaemia over 21 days using qPCR-detection of human DNA.

Methods CD1 nude mice (male, 6–8 weeks old; n=6 per group) underwent femoral artery ligation followed by injection with hESC-ECs into ischaemic muscle of the hindlimb. Mice were sacrificed at 0 hour, 4 hour, 24 hour, 7d, 14d, and 21d post-transplantation, and DNA extracted from the hind limb. A paired-qPCR assay was run with a set of human-specific and mouse-specific primers to allow for detection of hESC-ECs in mouse tissue.

Results A standard curve was constructed using mixtures of DNA extracted from mouse tissue and a population of hESC-ECs. The mean percentage of human cells at each time-point was determined accordingly. At the first time-point (0 hour), 1.77%±0.55% human cells were present within the ischaemic limb, with no significant change at 4 hour post-injection. However, by 24 hour and 7 days post-injection, the% human cells present significantly decreased to 0.67%±0.24% (p=0.05) and 0.35%±0.09% (p=0.05) respectively. At 14 and 21 days post-injection, the level of human DNA present had decreased to background levels.

Conclusion Our results suggest the majority of injected hESC-EC cleared from the injection site within the first 24 hours, with the remainder of the cells no longer present at 14 days post-injection. This is consistent with imaging data obtained prior to this study, and suggests that injected hESC-ECs improve perfusion in the mouse ischaemic limb by a paracrine mechanism rather than direct incorporation into the existing vasculature.

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