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Exercise modulates myocardial protein kinase B/Akt in Zucker obese rats
  1. B Huisamen,
  2. A Lochner
  1. Department of Medical Physiology and Biochemistry, Faculty of Medicine, University of Stellenbosch, Republic of South Africa
  1. Correspondence to:
    Dr Barbara Huisamen
    Department of Medical Physiology and Biochemistry, Faculty of Health Sciences, University of Stellenbosch, PO Box 19063, Tygerberg 7505, Republic of South Africa; bh3sun.ac.za

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In type 2 diabetes, heart muscle is insulin resistant. Protein kinase B (PKB)/Akt) acts as a mediator of the metabolic effects of insulin, including translocation of the insulin sensitive glucose transporter, GLUT4, in a manner dependent on phosphatidylinositol-3-kinase (PI-3-K).1 We have previously described dysregulation of PKB/Akt in hearts from a rat model of type 2 diabetes, the Zucker fa/fa rat.2

Exercise or contraction of heart muscle does not activate PKB/Akt or PI-3-K but can induce GLUT4 translocation and glucose uptake.3 However, exercise alleviates peripheral insulin resistance at a currently unknown, post-receptor level. To better understand the mechanism whereby this may be accomplished, we investigated whether chronic exercise will induce beneficial changes in the regulation of PKB/Akt that translates into improved myocardial insulin stimulated glucose uptake.

METHODS

To accomplish this, we subjected Zucker fa/fa rats (20 weeks old at the beginning of training) and a control group of age matched Wistar rats, to an exercise training programme, and documented changes in glucose uptake, myocardial GLUT4, and PKB/Akt expression and phosphorylation, in comparison to their sedentary counterparts. Rats were trained by swimming for four weeks, six days per week, on set times each day, starting at five minutes per day, escalating to 90 minutes per day, to avoid stress. The water temperature was 34°C and for half the exercise time, rats were weighted with 2% of their body weight, carried on their tails. Animals were sacrificed by intraperitoneal injection of sodium pentobarbitone (0.1 mg/g) after which ventricular myocytes were harvested via standard methods.4

2-Deoxy-D-glucose (2-DG) uptake by cardiomyocytes was measured as previously described4 while PKB/Akt phosphorylation and GLUT4 expression were determined via standard Western blotting techniques and suitable antibodies. PKB/Akt kinase activity was determined using a commercial non-radioactive assay system (Cell Signaling Technology). Laser scanning densitometry and suitable software (UN-SCAN-IT, Silkscience) were used to analyse the Western blots. Comparisons of datasets were made either by analysis of variance (ANOVA) followed by a Bonferroni correction or a Student’s t test for unpaired data. A probability value of p < 0.05 was considered significant.

RESULTS

Blood glucose determinations (Boehringer Mannheim, Accutrend glucose meter) at the time of euthanasia, demonstrated that Zucker fa/fa rats were diabetic at this age (mean (SEM) 10.3 (0.3) mmol/l (fa/fa) v 7.8 (0.4) mmol/l (Wistar), p < 0.01, n  =  6). Training lowered the blood glucose of the fa/fa group to 8.8 (0.6) mmol/l (p < 0.05, n  =  7).

2-DG accumulation by cardiomyocytes from sedentary diabetic rats (15.3 (1.4) pmol/mg protein/30 mins) was lower than the sedentary control group (25.5 (1.8) pmol/mg protein/30 mins), while insulin could not elicit a significant response in the former (19.9 (3.6) v 99.6 (8.7)). Exercise had no effect on basal glucose uptake (15.2 (1.7) pmol/mg protein/30 mins), but improved the insulin stimulated response to 38.5 (5.1) pmol/mg protein/30 mins in the diabetic rats. This was, however, still less than controls after exercise. Concurrently, we found a 30% lower GLUT4 content in diabetic versus control hearts. Expression of this protein was upregulated by exercise in the diabetic hearts only, to a level similar to that of control hearts after exercise.

We furthermore documented raised serine-473 phosphorylation of PKB/Akt in diabetic hearts compared to control hearts, under basal conditions. This was coupled to equal expression and kinase activity of the protein. Nevertheless, it was less sensitive to stimulation by insulin. Following training however, insulin stimulated phosphorylation of PKB/Akt was equal to that found in control hearts (fig 1).

Figure 1

 (A) Profile of serine-473 phosphorylation of PKB/Akt. (B) A representative Western blot of PKB/Akt serine-473 phosphorylation.

DISCUSSION

It is well established that exercise can improve insulin resistance by sensitising muscle to insulin mediated glucose metabolism, and it has been used to treat or prevent type 2 diabetes in patients successfully. Glucose transport is the rate limiting step in muscle glucose utilisation5 and the capacity for glucose transport may vary according to the total amount of GLUT4 protein. Exercise raised GLUT4 expression in diabetic hearts; however, despite this upregulation, insulin stimulated glucose uptake was still only 50% of control values, indicating either defective translocation of the transporter or glucose uptake per se.

A high level of serine-473 phosphorylation of PKB/Akt is usually associated with activation of the kinase and should lead to enhanced glucose uptake. However, we found high basal phosphorylation levels associated with low glucose uptake in sedentary diabetic animals. To the best of our knowledge, this is the first demonstration of such dysregulation at the level of PKB/Akt in diabetes. Exercise normalised the phosphorylation pattern of PKB/Akt in diabetic hearts, an effect probably caused by improved insulin signalling to the applicable phosphatases. This would mean that improved PKB/Akt phosphorylation is not the mediator of improved insulin resistance but an effect thereof. Furthermore, despite normal phosphorylation of this kinase in cardiomyocytes from trained diabetic rats and normalisation of GLUT4 expression, insulin stimulated glucose uptake was still curtailed. This result is an important indication that a major alteration in the diabetic heart, leading to faulty glucose metabolism, must be in the functioning or translocation of the GLUT4 protein. These results suggest the possibility that this signalling pathway is a potential target of treatment for type 2 diabetes.

REFERENCES

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