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Intracoronary photodynamic therapy reduces neointimal growth without suppressing re-endothelialisation in a porcine model
  1. R Waksman1,
  2. I M Leitch2,
  3. J Roessler2,
  4. H Yazdi1,
  5. R Seabron1,
  6. F Tio3,
  7. R W Scott2,
  8. R I Grove1,
  9. S Rychnovsky1,
  10. B Robinson1,
  11. R Pakala1,
  12. E Cheneau1
  1. 1Division of Cardiology, Washington Hospital Center, Washington, DC, USA
  2. 2Miravant Medical Technologies, Santa Barbara, California, USA
  3. 3The Biomedical Research Foundation of South Texas, San Antonio, Texas, USA
  1. Correspondence to:
    Dr Ron Waksman
    Washington Hospital Center, 110 Irving St, NW, Suite 4B-1, Washington, DC 20010, USA; ron.waksman{at}medstar.net

Abstract

Objective: To examine the effects of intracoronary PhotoPoint photodynamic therapy (PDT) with a new photosensitiser, MV0611, in the overstretch balloon and stent porcine models of restenosis.

Methods: 28 pigs were injected with 3 mg/kg of MV0611 systemically 4 h before the procedure. Animals were divided into either the balloon overstretch injury (BI) group (n  =  19) or the stented group (n  =  9). After BI, a centred delivery catheter was positioned in the artery to cover the injured area, and light (532 nm, 125 J/cm2) was applied to activate the drug (n  =  10). Control arteries (n  =  9) were not activated by light. In the stented group, the drug was light activated before stent deployment. Serial sections of vessels were processed 14 days after treatment in the BI group and 30 days after treatment in the stented group for histomorphometric or immunohistochemical analysis.

Results: Intracoronary PDT significantly reduced intimal thickness in both BI and stented arteries (about 65%: 0.22 (SEM 0.05) mm v 0.62 (0.05) mm, p < 0.01; and about 26%: 0.40 (0.04) mm v 0.54 (0.04) mm, p < 0.01, respectively). PDT increased luminal area by ⩽ 60% and 50% within BI and stented arteries (3.43 (0.27) mm2v 5.51 (0.52) mm2, p < 0.05; 4.0 (0.02) mm2v 6.0 (0.16) mm2, p < 0.01), respectively. Complete re-endothelialisation was observed by immunohistochemical and gross histological analyses in all PDT and control arteries. There were no cases of aneurysm formation or thrombosis.

Conclusion: Intracoronary PhotoPoint PDT with MV0611 reduces intimal proliferation without suppressing re-endothelialisation in a porcine model of restenosis.

  • BI, balloon overstretch injury
  • PDT, photodynamic therapy
  • SMC, smooth muscle cell

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Footnotes

  • Published Online First 17 March 2006

  • Miravant Medical Technologies supported this study. Advanced Cardiovascular Systems, Inc, a subsidiary of Guidant Corporation, supplied stents for this study. PhotoPoint is a trade name of Miravant Medical Technologies, Santa Barbara, California, USA.

  • Competing interests: None declared.

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