Objective To study the effects of CSWT on the proliferation, differentiation of endothelial progenitor cells (EPCs) from peripheral blood in patients with coronary heart disease (CAD).
Methods Total mononuclear cells were isolatedfrom patients with coronary heart disease (n=25) and age-matched control subjects (n=25) by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin coated culture dishes. After 7 days of culture. EPCs were characterised as adherent cells double positive for DiLDL-uptake and lectin binding by direct fluorescent staining under alaser scanning confocal microscope.
Results The number and proliferation of EPCs was significantly increased in patients CSWT as compared with that in control subjects (p<0.05), CSWT could markedly promote the proliferation and the differentiation towards the endothelial cell lineage of EPCs in CAD.
Conclusion CSWT could markedly promote the proliferation and the differentiation towards the endothelial cell lineage of EPCs in CAD.