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Basic science: Cardiovascular disease basic research
e0035 The study of derivation from bone marrow mesenchymal stem cells into cardiomyocyte-like cells in vitro via cardiotrophin-1
  1. Chaoquan Peng,
  2. Ya Gao,
  3. Peng Xiang,
  4. Ke Yang,
  5. Liyuan Zou,
  6. Xiao Wu
  1. The Third Afiliated Hospital of Sun Yat-sen University

Abstract

Objective To investigate the effects of CT-1 on differentiation of induced swine BMSCs in vitro independently or with 5-aza.

Methods BMSCs were divided into four groups : bland contro1; induced with CT-1; induced with 5-aza; induced with 5-aza combined with CT-1. After 4 weeks of induced culturing, the differentiation of cardiomyocyte induced were estimated by cTnT and a-actinin.

Result Red fluorescence staining of a-actin shows: the differentiation rate of myocardial cells of the composite group is greatest (29.900±4.7571%); 5-aza group is greater than CT-1 group; CT-1 group is greater than control. Red fluorescence staining of cTnT shows: the differentiation rate of myocardial cells of the composite group is greatest (36.500±4.0927%); 5-aza group is greater than CT-1 group; CT-1 group is greater than control.

Conclusions CT-1 can increasing the inducing rate commbined witn 5-aza.

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