Objective To explore the significance of the ACE gene insertion/deletion (I/D) polymorphism in peoples of Hainan Li and Han nationality with coronary heart disease (CHD).
Methods Used the PCR to detect the polymorphisms of ACE gene insertion/deletion (I/D) in 150 patients with CHD and 150 healthy people from Hainan Han and Li nationality respectively. Observed the genotype frequencies and allele frequencies of DD, DI and II. Specific PCR detection was performed for patients who have been determined as DD by normal PCR to reduce misclassification rate. Meanwhile detected the blood lipid, the lipoprotein, the blood pressure, the blood sugar in all people. Used the multiple regression analysis to find out the risk factor in CHD patients.
Results The genotype frequencies of DD in the CHD group of Han and Li nationality are significantly higher than the control group of Han and Li (p<0.05). There were no significant differences in the genotype frequencies of DD, DI and II between the Han and Li with CHD. By the multiple regression analysis it shows: the genotype frequencies of DD in the CHD group of Han and Li nationality increased, the high density lipoprotein cholesterol (HDL-C) level in the CHD group of Han and Li nationality decreased. The triglyceride (TG) level in the CHD group of Han nationality increased.
Conclusions The genotype frequencies of DD are associated with CHD. The susceptibility of CHD in Han and Li nationality is the same. Increasing the HDL-C level can protect the CHD patients. The high level of TG is the independent risk factor in Hainan Han people with CHD.
- Coronary heart disease
- ACE gene