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Clinical and research medicine: Coronary heart disease
e0401 Changes of intracellular calcium concentration in cardiac-like myocytes
  1. Wang bo,
  2. Bai xiaojuan
  1. The First Affiliated hospital of China Medical University

Abstract

Objective To study the effects of verapamil, endothelin on [Ca2+]i in cardiac-like myocytes derived of bone marrow mesenchymal stem cells.

Methods (1) Bone marrow mesenchymal stem cells and cardiac myocytes were cultured by primary method; (2) Bone marrow mesenchymal stem cells differentiated into cardiac-like myocytes by 5-azacytidine induction agent; (3) We divide our test into three groups, including the first generation bone marrow mesenchymal stem cells, cardiac-like myocytes and cardiac myocytes; (4) The alteration of [Ca2+]i affected by verapamil, endothelins was observed with [Ca2+]i fluorescence imagine system in bone marrow mesenchymal stem cells, cardiac-like myocytes and cardiac myocytes.

Results (1) Cardiac myocytes were set up through trypsogen digestion method; (2) The alteration of [Ca2+]i affected by verapamil was observed with [Ca2+]i fluorescence imagine system. There was a same trend of variability between cardiac-like myocytes and cardiac myocytes, but no changes in bone marrow mesenchymal stem cells; (3) The alteration of [Ca2+]i affected by endothelin was observed with [Ca2+]i fluorescence imagine system. There was a same trend of variability that fluorescence intensity gradually strengthened with intervention time extended.

Conclusions After affected by verapamil and endothelins, there was a same trend of [Ca2+]i changes between cardiac-like myocytes from bone marrow mesenchymal stem cells and cardiac myocytes. It shows that some common electrophysiological characteristics exist in cardiac-like myocytes and cardiaomyocytes.

  • Bone marrow mesenchymal stem cells, cardiac-like myocytes, cardiomyocytes
  • [Ca2+]

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