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Basic science: Cardiovascular disease basic research
e0038 Effects of Angiotensin II on ATPases in aortic vascular smooth muscle cells from Wistar-Kyoto rats and spontaneously hypertensive rats
  1. Qian-Hui Shang1,
  2. Gui-Hai Zhang1,
  3. Qian-Feng Jiang1,
  4. Wei-Hong Wan2
  1. 1Institute of Clinical Medicine, Zunyi Medical College, Department of Cardiology, Affiliated hospital of Zunyi Medical College
  2. 2Key laboratory of cell engineering of Guizhou Province, Zunyi, Guizhou, China

Abstract

Aim To explore the effects of Angiotensin II on the activities of Ca2+-ATPase, Na+, K+-ATPase and mRNA expression levels of the plasma membrane Ca2+-ATPase isoform 1 (PMCA1) and Na+, K+-ATPase α1-subunit in cultured thoracic aortic vascular smooth muscle cells (AMSCs) from Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR).

Methods AMSCs isolated from 14-week-old male WKY rats and SHR were cultured and treated with different concentrations (1×10−9, 1×10−8, 1×10−7 mol/l) of Angiotensin II. The activities of Ca2+-ATPase, Na+, K+-ATPase were measured by biochemistry and enzymology. RT-PCR assay was employed to determine the relative levels of PMCA1 and Na+, K+-ATPase α1-subunit mRNA in AMSCs.

Results Low and moderate concentration of Angiotensin II significantly increased the activity of Ca2+-ATPase and up-regulated PMCA1 mRNA level in AMSCs from Wistar-Kyoto rats, while high concentration of Angiotensin II inhibited Ca2+-ATPase activity and down-regulated PMCA1 mRNA level. Three different concentration of Angiotensin II significantly decreased the activity of Ca2+-ATPase and PMCA1 mRNA level in AMSCs from SHR. Three different concentrations of Angiotensin II stimulated the activity of Na+, K+-ATPase and increased its α1-subunit mRNA expression in AMSCs from WKY rats. Low and moderate concentration of Angiotensin II did not affect the activity of Na+, K+-ATPase in SHR, while high concentration of Angiotensin II significantly suppressed the activity and α1-subunit mRNA level of Na+, K+-ATPase.

Conclusions In WKY rats, Angiotensin II may have biphasic effects on Ca2+-ATPase activity and PMCA1 mRNA expression, and may promote the activity and α1 subunit mRNA expression of Na+, K+-ATPase in a dose-dependent manner in AMSCs. In SHR, Angiotensin II can inhibit Ca2+-ATPase activity and PMCA1 mRNA expression, and only high dose of Angiotensin II can suppress the activity and α1 subunit mRNA expression of Na+, K+-ATPase in AMSCs.

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