Article Text

PDF

Clinical and research medicine: Acute coronary syndrome
e0464 Clinical significance of the changes of serum TNF-α, IFN-γ, MMP-9
  1. Zhang Zhaozhi,
  2. Meng Xiaoping
  1. Cardiology The Second Hospital of Jilin University

Abstract

A variety of inflammatory factors in atherosclerosis (AS) plays a complex role, the combination of two or more of the inflammatory mediators can increase inflammatory mediators in coronary heart disease diagnosis and treatment of value.

Objective To investigate Clinical significance of the changes of serum TNF-α, IFN-γ, MMP-9, oxLDL levels in patients with acute coronary syndrome (ACS).

Method Selected 63 patients who had been done coronary angiography from June 2009 to January 2010 in our hospital. There are 37 males and 26 females, divided into 3 groups: acute myocardial infarction (AMI) group, unstable angina group and the normal control group. According to the results of coronary angiography the patients were divided into 3 groups: acute myocardial infarction group, unstable angina group and the normal control group. There are 27 cases in AMI group, 17 males and 10 females, mean age (62.5±11.4) years. Selection criteria: According to clinical symptoms, ECG changes, myocardial enzyme increases, coronary angiography, all patients are Q-wave myocardial infarction, and the incidence in the 3h∼24h. There are 20 patients in UAP group, 13 males and 7 females, mean age (64.4±9.1) years of age. Selection criteria: Overworked deterioration of angina and (or) resting angina, and at least onseted once severe angina in 48h, electrocardiographic shows ischaemic changes, myocardial enzymes normal, coronary angiography showed at least a coronary artery stenosis degree >70%. The normal control group, there are 22 cases, 12 males and 10 females, mean age (60.8±9.4) years of age. Selection criteria: chest pain, ECG and myocardial enzymes normal, coronary angiography showed coronary artery no stenosis. AMI and UAP patients, blood were taken 5 ml immediately after admission, the normal control group blood were taken 5ml in the next morning. The blood serum was obtained after centrifugated on the same day and placed in −20°C in refrigerator to preserve. Plasma concentrations of TNF-α, IFN-γ, oxLDL, MMP-9 were measured in a day. Using enzyme-linked immunosorbent test (ELISA) test, kit were bought from Adlitteram Dignostic Laboratories, Inc in USA. The operating instructions strictly according to the kit. To read each hole OD value through the length 450 nm microplate. The sample values were taken into four parameters OD fitting formulae, calculated samples density. Use SPSS14.0 statistical software applications to analyse, measurement data using x¯±s, measurement data use t-test, count data using χ2 test, p<0.05 for significant difference. Many factors applicate Logistic model do correlation analysis.

Results The plasma level of TNF-α in AMI group and UAP group, no statistical significance (p>0.05), AMI group compared with the control group the serum levels of TNF-α increased significantly (p<0.05), UAP group compared with the control group the serum levels of TNF-α increased significantly (p<0.05). The plasma level IFN-γ AMI group compared with UAP group, no statistical significance (p>0.05), AMI group compared with the control group the serum levels of IFN-γ increased significantly (p<0.05), UAP group compared with the control group the serum levels of IFN-γ increased significantly (p<0.05). MMP-9 plasma levels AMI group compared with UAP group the serum levels of MMP-9 increased significantly (p <0.05), AMI group compared with the control group the serum levels of MMP-9 increased significantly (p<0.05), UAP group compared with the control group the serum levels of MMP-9 increased significantly (p<0.05). The plasma levels of oxLDL AMI group compared with UAP group, no statistical significance (p>0.05). AMI group compared with the control group the serum levels of oxLDL increased significantly (p<0.05). UAP group compared with the control group the serum levels of oxLDL increased significantly (p<0.05).

Conclusion The plasma levels of TNF-α, IFN-γ, MMP-9 and oxLDL in patients with acute coronary syndrome are significant positive correlation. The plasma levels of TNF-α, IFN-γ, MMP-9 and oxLDL can be used to determine the nature of coronary atherosclerotic plaques. The plasma levels of MMP-9 increased hints plaques instability, Also can be used as an important biochemical monitoring index about the risk stratification of coronary artery disease.

  • TNF-α
  • IFN-γ
  • MMP-9
  • oxLDL
  • acute coronary syndrome (ACS)

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.