Objective To investigate whether the role of Ginsenosides-Rbl (Gs-Rb1) on aldosterone-induced elastic fibre deposition of neonatal rat cardiac fibroblasts (CFs) in vitro.
Methods CFs were randomly divided into control group, aldosterone group (10 nmol/l), Gs-Rb1 group (200 umol/l) and Gs-Rb1 binding aldosterone group (100, 200, 300, 400, 500 umol/l Gs-Rb1, respectively, basing on 10 nmol/l aldosterone), all of which were treated for 24 h. MTT colorimetric assay was adopted to evaluate cell proliferation whereas immunofluorescence cytochemistry and western blot were used to detect elastin, tropoelastin synthesis and elastic fibre deposition.
Results 1. Gs-Rb1 significantly inhibited CFs proliferation induced by aldosterone in a dose-dependent manner (p<0.01). 2. Aldosterone significantly increased elastic fibre deposition, the expression of elastin (p=0.024) and tropoelastin collagen (p=0.031) in CFs. 3. Pretreatment with Gs-Rb1 significantly inhibited the above aldosterone effects, including elastin levels (p<0.01), tropoelastin synthesis (p<0.01) and elastic fibre deposition in a dose-dependent manner.
Conclusions Gs-Rb1 was shown to inhibit aldosterone-induced collagen production in CFs.