Objectives The purpose of this study was to establish an rat model of CIN and to evaluate its efficacy.
Methods Totally 24 SD rats were randomly allocated into experimental group (group A, n=12) and control group (group B, n=12). After dehydration for 3 days, rats in group A were given intravenous MDDS, while rats in group B were given intravenous normal saline. Then, all rats got normal water-drinking to the end of study. Renal ultrasonic examination was performed to observe the morphologic changes, diameters of renal artery and blood flow in renal artery. Blood samples were taken to measure the level of serum creatinine. The tissue of kidney were incised for microscope and electron microscope study.
Results The dimensions of the two groups before and after dehydration were not different. It gradually enlarged after CM injection. These changes were the most obvious at 6 and 12 h, which did not recover at 24 h. The PSV, EDV, S/D and VTI were lowerest at 6 h and then recover to normal level at 24 h. RI was increased after CM injection, the lowest occurred at 6 h, and recovered to normal level at 24 h. Serum creatinine was significantly elevated after dehydration, the highest level occurred at 12 h and then began to recover at 24 h. Microscope examination to renal sample at 12 h found patch disappearance of tubular structure, widely congestion at medullar area. No pathological glomerular changes were found under microscope. Electron microscope examination found desquamation, sparseness of microvillous of tubular endothelium, membrane confusion, disappearance, swelling, fragmentation of the MIT, with obstructed tubular lumen and basal membrane swelling.
Conclusion Combined with dehydration, intravenous injection of contrast lead to obvious acute kidney injury, with the changes of kidney tissue pathology, haemodynamics and kidney functions which are similar to the characteristics of CIN in human beings.