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Basic science: Cardiovascular disease basic research
e0005 Model of cardiac arrest in rats by transcutaneous electrical epicardium stimulation
  1. Lin Ji-Yan,
  2. Li Xin,
  3. Li Hui,
  4. Wei Hong-Yan,
  5. Liu Rong,
  6. Hu Chun-Lin,
  7. Dai Gang,
  8. Liao Xiao-Xing
  1. The First Affiliated Hospital of Sun Yat-sen University

Abstract

Objective To establish a new model of Cardiac Arrest (CA) in rats by transcutaneous electrical epicardium stimulation.

Methods Two acupuncture needles connected to the anode and cathode of a stimulator were transcutaneously inserted into the epicardium as electrodes. The stimulating current was steered to the epicardium and the stimulation was maintained for 3 min to induce CA. Cardiopulmonary resuscitation (CPR) was performed at 6 min after a period of nonintervention.

Results The success rate of induction was 12/20 at the current intensity of 1 mA; and reached 20/20 when the current intensity was increased to 2 mA. The average time from the electrical stimulation to CA induction was 5.10 (±2.81) s. When the electrical stimulation stopped, 18/20 rats had ventricular fibrillation and 2/20 rats had pulseless electrical activity. CPR was performed for averagely 207.4 (±148.8) s. The restoration of spontaneous circulation was 20/20. The death rate within 4 h after CA was 5/20, and the 72-h survival rate was 10/20. There were only two cases of complications, a minor muscle contraction and a minor lung lobe injury.

Conclusion The model of CA in rats induced by transcutaneous electrical epicardium stimulation is a stable model that requires low-intensity current and has fewer complications.

  • Cardiac arrest
  • cardiopulmonary resuscitation
  • epicardium
  • electrical stimulation
  • animal model

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