Objective Anisodamine is a M-cholinergic receptor inhibitor that plays improvement effectiveness on the microcirculative perfusion. Our previous study had ever shown reversing effect on no-reflow phenomenon (NRP) in the miniswine models with AMI. The purpose of this study was continually to explore the effect of preventive intracoronary administration of anisodamine on myocardial microcirculation in York swines models with AMI by TIMI frame count, TIMI myocardial perfusion grade and haemodynamic parameters.
Methods 18 York swines (25∼35 kg, 3∼4 months old) were divided into saline group (n=9) and Anisodamine group (n=9). Immediately after 2 ml saline and 2 mg anisodamine were injected into LAD in the two groups respectively, PMBS were injected into the coronary artery by three times with 5 min interval, then incidence of NRP was recognised by TIMI frame count and TIMI myocardial perfusion grade. TnI and CK-MB were measured before PMBS injection and at 5, 60, 120, 180 min after PMBS injection. MPP and PCWP were measured by Swan-Ganz catheter. ECG was recorded before and immediately after PMBS injection and ΣST were calculated. The changes of PR, QRS and QT duration were also compared.
Results 16 of 18 York swines survived in the whole procedure. NRP was found in all of the eight swines in saline group while four of eight swines in anisodamine group. MPP (mean perfusion pressure) was slightly increased by 4.76% only in anisodamine group (p>0.05), while MPP were significant decreased by 25.23%, 23.63%, 19.66 % immediately, 3 min and 1 h after NRP in saline group (118.5±16.2 vs 88.6±12.3, 118.5±16.2 vs 90.5±14.3, 118.5±16.2 vs. 95.2±16.3 mm Hg, p<0.05), respectively, and PCWP, values of ΣST and mycardial Infarct size in anisodamine group were much lower than that in saline group significantly (p<0.05).
Conclusion Preventive intracoronary administration of anisodamine could improve AMI survival rate with NPR by maintaining effective myocardial microcirculation status and raising coronary perfusion pressure as well decreasing the size of myocardial infarction.