Objective To investigate effect of bone marrow mesenchymal stem cells transplantation on expression of nuclear factor κB (NF-κB) and proliferating cell nuclear antigen (PCNA) and vascular stenosis after carotid artery balloon injury of rabbit.
Methods 36 carotid artery atherosclerotic stenosis rabbits were randomly divided into the control group (balloon injury+PBS solution) and the MSCs transplantation group (balloon injury+MSCs transplantation). MSCs (5×107/ml) were pre-labelled by DAPI and then infused into MSCs transplantation group rabbits by the ear vein, and control group was infused with the same amount of PBS solution. 1 week after MSCs transplantation, DAPI labelled cells were detected under immunofluorescence microscope; The plasma tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were detected with ELISA on the 1st, 2nd and 4th week after MSCs transplantation. After 2 weeks and 4 weeks, the injured vessels were stained by HE and the immunohistochemical analysis of NF-κB and PCNA.
Results The DAPI-labelled MSCs could be detected on impaired intimae 1 week after MSCs transplantation. NF-κB and PCNA expression was not seen in the normal blood vessels after 2 weeks, the expression of NF-κB and PCNA in MSCs transplantation group decreased significantly compared with control group. The plasma TNF-α and IL-6 levels in MSCs transplantation group were significantly lower than those in control group. The intimal area, the ratio of the intima/media area and the luminal stenosis ratio were significantly lower in MSCs transplantation group than control group at 4 weeks.
Conclusions MSCs are capable of decreasing the inflammatory reaction of injured vessels and lighten the restenosis of injured vessels.
- Mesenchymal stem cells
- vascular stenosis