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Basic science: Cardiovascular disease basic research
e0090 Effect of granulocytemacrophage colony stimulating factor on coagulation and fibrinolysis in rabbit model with iliac damaged by balloon
  1. Ma Xiaojing,
  2. Zhang Xinghua
  1. Provincial Hospital Affiliated To Shandong University

Abstract

Objective Endothelial dysfunction after percutaneous coronary intervention (PCI) played a key role in perturbations of haemostatic equilibrium. Granulocyte-macrophage colony stimulating factor (GM-CSF) could enhance the restoration of cells' normal phenotype. However, previous studies suggested GM-CSF might induce hypercoagulability. In this study, we discussed the effect of GM-CSF on coagulation and fibrinolysis after artery deendothelialization.

Methods 24 male New Zealand White rabbits underwent primary iliac artery deendothelialization were randomised to two groups (GM-CSF group and control group). GM-CSF group animals received a subcutaneous injection of GM-CSF, the control group animals were given a subcutaneous injection of equivalent saline. The iliac arteries of all animals were damaged by balloon after 7 days. The plasma levels of tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1) and nitric oxide (NO) were detected before, 1 week, 2 weeks and 4 weeks after angioplasty. The repair and hyperplasia of intima were observed by microscope.

Results The NO levels of the GM-CSF group were higher than that of the control group 2 weeks and 4 weeks after operation [(91.9±11.6) μmol/l vs (81.7±12.2) μmol/l]; [(97.7±10.1) μmol/l vs (83.2±12.6) μmol/l]. Although there was no difference between the two groups in both activities of t-PA and PAI-1 in each specific time point, in 1, 2, 4 weeks after operation, the activities of t-PA became higher than the ones before operation, while the activities of PAI-1 stayed almost the same. Pathological examination showed that the level of intima hyperplasia. VSMC and fibrous tissue of neointima were much lower in GM-CSF group and endothelium was more integrated and smooth.

Conclusion GM-CSF could facilitate the repair of intima, better the function of endothelium without disturbing the balance of coagulation and fibrinolysis.

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