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Basic science: Cardiovascular disease basic research
e0102 Expression or secretion of IL-34 and IL-35 in the peripheral blood mononuclear cells from patients with dilated cardiomyopathy
  1. Huang Sisi,
  2. Wu Weifeng,
  3. Lin Song,
  4. Huang Yanlan
  1. The First Affiliated Hospital of Guangxi Medical University

Abstract

Objective The aim of this study was to observe the level of interleukin (IL)-34 and IL-35 in peripheral blood mononuclear cells (PBMCs) with dilated cardiomyopathy (DCM), and explore the role of IL-34 and IL-35 in human DCM.

Methods 30 patients with DCM and 30 normal adults as control were studied. IL-34 and the subunit Epstein-Barr virus-induced gene 3(EBI3) of IL-35 mRNA expression in PBMCs were detected by reverse transcription–PCR (RT-PCR). IL-34 and IL-35 protein level in plasma were measured with ELISA.

Result (1) Results showed that the IL-34 mRNA level or its protein level was significantly elevated in DCM patients compared with normal control subjects. (mRNA level: DCM :0.37±0.08, NC: 0.19±0.03, p<0.01; protein level: DCM: 208.94±50.31 pg/ml; NC: 175.69±44.56 pg/ml; p<0.01) (2) The IL-35 subuint-EBI3 or its protein level was significantly decreased in DCM patients compared with normal control subjects. (EBI3 mRNA level: DCM: 0.15±0.03, NC: 0.33±0.07, p <0.01; protein level: DCM: 128.68±24.08 pg/ml, NC: 179.73±43.89 pg/ml, p<0.01) (3) The secretion of IL-34 was markedly correlation with the secretion of IL-35 (r=−0.490, p <0.01). (4) The protein level of IL-34 in DCM patients had a positive correlation with heart function (r=0.598, P <0.01). (5) The protein level of IL-35 in DCM patients had a negative correlation with heart function (r=−0.839, p<0.01).

Conclusion The ability to express IL-34 and IL-35 protein or mRNA in PBMCs is abnormal and the change strongly correlates with ejection fraction and heart function of DCM patients.

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