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Basic science: Cardiovascular disease basic research
e0106 The effect of adenosine and ischaemia postconditioning on MMP-2 and MMP-9 in rabbit ischaemia reperfusion myocardial
  1. HeXiaonan1,
  2. Yu Chen2,
  3. Li Shumei3
  1. 1Department of Cardiology, TianJin Chest Hospital, TianJin
  2. 2Department of Cardiology, China—Japan Union Hospital, Jilin University, Changchun
  3. 3Department of Cardiology, the Second Hospital of Jili University, Changchun

Abstract

Objective To observe the effect of adenosine and ischaemia postconditioning on MMP-2 and MMP-9 in rabbit ischaemia reperfusion myocardial.

Methods The rabbits were divided into four groups in basic experiment: control group, antagon group, postconditiong group and adenosine group. The activity of MMP-2 and MMP-9 was observed by gelatin zymography and the expression of MMP-2 and MMP-9 was observed by RT-PCR and Western Blot.

Results The results of RT-PCR showed that the light density of MMP-2/GAPDH (0.76±0.22) in adenosine group and postconditioning group was slightly lower than that of control group and antagon group0. 80±0. 20 (p>0.05). The light density of MMP-2/GAPDH (0.77±0.12) in adenosine group and postconditioning group was significanty lower than that of control group and antagon group 1.30±0. 10 (p<0.01). There was no dramitic difference between adenosine group and postconditioning group. The results of Western blot showed that MMP-9 and MMP-2 in adenosine group and postconditioning group was lower than control group and antagon group. The results of Zymography revealed that the light density of MMP-9 in adenosine group and postconditioning group much lower than those in control group and antagon group (p<0.05). There was no difference between adenosine group and postconditioning (p>0.05).

Conclusions Adenosine and postconditioning can decrease the expression and the activity of MMP-9 and inhibit the inflammation, relieving the ischaemia reperfusion injury.

  • Adenosine
  • MMP-2, MMP-9, Myocardial reperfusion injury

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