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Basic science: Cardiovascular disease basic research
e0114 Expression of pregnancy-association plasma protein A and inducible nitric oxide synthase in the wall of balloon injured and early atherosclerotic porcine coronary artery
  1. Weiyi Mei,
  2. Zhimin Du,
  3. Chengheng Hu,
  4. Guifu Wu,
  5. Yi Li,
  6. Chufang Luo
  1. Department of Cardiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

Abstract

Purpose To investigate the role of pregnancy-associated plasma protein A (PAPP-A), a novel marker of atherosclerotic plaque activity, in the progress of injured-restenosis and atherosclerosis, and the relationship between the expression of PAPP-A and inducible nitric oxide synthase (iNOS) in the wall of coronary artery.

Methods The balloon injury procedure was done in the coronary arteries of 5 male pigs (injury group), and the artery segments were harvested in 28d after balloon injury. The expression of PAPP-A and iNOS were detected in the wall of coronary arteries by the means of immunohistochemical study and reverse transcription-polymerase chain raction. Expression of PAPP-A and iNOS were also detected in coronary artery wall of four pigs fed a high-cholesterol atherogenic diet for 15 weeks (CHOL group).

Results A marked increase in PAPP-A-positive cell number of the injury group was seen compared with the CHOL group, both in medial smooth muscle cells (PAPP-A staining: 33.2±2.9 vs 5.5±2.8, p<0.05) and neointimal (intimal) cells (PAPP-A staining: 28.3±3.1 vs 3.8±2.4, p<0.05); while iNOS-positive cell number decrease, only in neointimal (intimal) cells (iNOS stain: 1.1±0.3 vs 18.4±4.2, p<0.01). The expression of PAPP-A mRNA was higher in the injury group, compared with the CHOL group (0.81±0.08 vs 0.54±0.13, p<0.05), but nearly no expression in “normal” control vessel segements (0.03±0.01); while iNOS mRNA was lower in the injury group (0.18±0.09 vs 0.62±0.13, p<0.05).

Conclusion PAPP-A plays role in the progress of early atherosclerotic lesions and restenostic lesions.

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