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Basic science: Cardiovascular disease basic research
e0121 The effects of endothein-1 and BQ-123 on ATPase activity and mRNA expression in aortic smooth muscle cells from spontanously hypertensive rats
  1. Qian-Hui Shang1,
  2. Zheng Yang1,
  3. Qian-feng Jiang1,
  4. Yu Xiao2
  1. 1Institute of Clinical Medine of Zunyi Medical College, Department of Cardiology, Affiliated Hospital of Zunyi Medical College, Guizhou, China
  2. 2Key Laboratory of Cell Engineering of Guizhou Province, Zunyi, Guizhou, China

Abstract

Aim To study the effects of endothelin-1 (ET-1) and BQ-123 (ETA receptor antagonist) on activities and mRNA expression of ATPase in aortic smooth muscle cells (ASMCs) from spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats.

Methods The ASMCs were isolated from SHR and WKY rats. The ATPase activities of cultured ASMCs were determined by spectrophotography. The mRNA levels of Na+, K+-ATPase α1-subunit and plasma membrane Ca2+-ATPase isoform 1 (PMCA1) were measured by semiquantitative reverse transcription PCR (RT-PCR).

Results 3 different concentrations of ET-1 (1×10−9, 1×10−8 and 1×10−7 mol/l) significantly attenuated the activities of Na+, K+-ATPase and Ca2+-ATPase and PMCA1 mRNA expression (all p<0.01) in ASMCs from SHR. Three different concentrations of BQ-123 (1×10−8, 1×10−7 and 1×10−6 mol/l) obviously prevented ET-1 mediated the inhibition of two kinds ATPase activities (all p<0.01) and downregulation of PMCA1 mRNA expression (p<0.01). But the mRNA expression level of Na+, K+-ATPase α1-subunit had no alteration after intervened by ET-1 (p>0.05).

Conclusions ET-1 may suppress Na+, K+-ATPase, Ca2+-ATPase activities via ETA receptor. The influence of ET-1 on Ca2+-ATPase activity may partially occur in the transcriptional level. BQ-123 can inhibit the effect of ET-1 on two kinds ATPase activities of ASMCs in SHR by blocking the ETA receptor.

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