Objective To establish a method for determination of pulmonary artery pressure and cardiac output in rat.
Methods 20 Sprgue—Dawely rats were randomly assigned into two groups: control group and pulmonary arterial hypertension (PAH) group. Rats in PAH group were received a single subcutaneous injection of monocrotaline (60 mg/kg). The hand-made PE-50 catheters were inserted into pulmonary artery via right jugular vein, which we can perform mean pulmonary artery pressure. Similarly, cardiac output was detected through thermodilution method.
Results After 21 days, compared with control group, mean pulmonary artery pressure was significantly increased (17.4±1.8 mm Hg in control group vs 61.8±4.3 mm Hg in PAH group, respectively) and cardiac output was significantly decreased (130±5.8 ml/min in control group vs 71±6.7 ml/min in PAH group, respectively) in PAH group.
Conclusions This method is a simple and direct method to detect pulmonary artery pressure and cardiac output in rat.
- Mean pulmonary artery pressure
- cardiac output
- right heart catheterisation