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Basic science: Experiment research
e0176 The effects of rosuvastatin on the expression of homocysteine-induced expression of matrix metalloproteinase-2 (MMP-2) and cell migration in rat vascular smooth muscle cells
  1. Yangbo Xing,
  2. Hangyuan Guo,
  3. Yafei Shi
  1. Department of Cardiology, Shaoxing People' Hospital, Shaoxing, China

Abstract

Objective The aim of this study was to investigate the effects of rosuvastatin on the expression of homocysteine-induced expression of matrix metalloproteinase-2 (MMP-2) and cell migration in rat vascular smooth muscle cells (VSMC).

Methods Cultured rat VSMC were incubated with different concentrations of Hcy and rosuvastatin (Hcy 1000 μmol/l) in vitro for 24, 48 and 72 h. The expression of MMP-2 was determined by using the methods of gelatin zymography and western blotting. Cultured rat VSMC was incubated with different concentrations of Hcy and rosuvastatin (Hcy 1000 μmol/l) in transwell for 24, 48 and 72 h. The number of VSMC which transited the membrane represented the aggressivity of VSMC.

Results Hcy (50∼1000 μmol/l) increased the expression and activity of MMP-2 significantly. Incubated with the same concentration of Hcy the expression and activity of MMP-2 of 72 h was higher than that of 24 h and 48 h. Hcy reduced the expression of MMP-2 at the concentration of 5000 μmol/l. Rosuvastatin could inhibit the augmentation of homocysteine-induced expression and activity of MMP-2. Hcy (50∼5000 μmol/l) could stimulate the migration of VSMC. Rosuvastatin could decrease the stimulation of homocysteine-induced migration of VSMC.

Conclusions These data suggested that Hcy can increase the MMP-2 expression/activity and the migration of VSMC. It may be one of the roles in the pathogenesis of atherosclerosis induced by Hcy. Rosuvastatin can inhibit the augmentation of homocysteine-induced MMP-2 expression/activity and migration of VSMC. This may be one of the pleiotropic of rosuvastatin besides lipid-lowering and benefit the therapy of CHD.

  • Homocysteine
  • rosuvastatin
  • matrix metalloproteinase-2
  • cell migration

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