Objective To explore and develop one optimise method that it could establish the porcine model of acute myocardial infraction more safer, quicker, convenient than routine methods.
Methods 30 animals with health condition, mean weight 26.5±4.8 kg; The pigs were divided into two groups randomly, group A (n=13) and group B (n=17), according to different method. Angioplasty balloon was positioned in the mid-distal of left anterior descending (LAD). The full balloon was inflated and occluded the LAD for 60 min after ischaemia precondition in animals of group A, and then the balloon embolism was positioned in the target vessel; but in group B, the balloon embolism was positioned in the target vessel directly. Intervention operation times and success rate were observed and compared with two groups.
Results 27 pigs underwent induction of AMI successful. Three pigs died of ventricular fibrillation and shock. Success rate of group A was 84.6%, and that of group B was 94%, there was not statistic significant compared with two groups. But mean operation time of group B (28.4±9.4 min)was shorter than that of group A (105.8±27.6 min), furthermore, compared with two groups, there was statistic significant.
Conclusions The method of establishment closed chest porcine model of AMI by implantation balloon embolism in target vessel is feasible, safe, quick and relatively effective.