Objective To investigate the effect of different methods of extracorporeal cardiac shock wave therapy on angiogenesis and expression of VEGF in acute myocardial infraction pigs, and optimise that of methodology.
Methods 25 miniature swine were randomly divided into three groups: group of cardiac shock wave therapy (n=15), positive control group (n=5), negative control group (n=5). According to the method, the animals of cardiac shock wave therapy were divided three subgroups: standard, prolonged course of treatment and extend area. The number of capillary density, mRNA of VEGF were evaluated and compared between with every group.
Results Compared with control positive group, the number of capillary density (837±54 vs 1856±78, p<0.0001) and expression mRNA of VEGF (20.52±4.94 vs 28.56±6.84) increased in the group of cardiac shock wave therapy, especially, in group of prolonged course of treatment. Whereas, there was no significance in the difference of between standard group and extend area group in capillary density (1633±24 vs 1695±32/mm2, p>0.05) and mRNA of VEGF (26.31±7.24 vs 27.44±3.59, p>0.05).
Conclusions Successive extracorporeal cardiac shock wave therapy at early stage of acute myocardial infarction could improve myocardial micro-vascular circulation. It will be a new and non-invasive angiogenic therapy.