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Basic science: Experiment research
e0214 N-acetylcysteine inhibits oxidised LDL, metalloproteinases -9 sercretion and apoptosis in atherosclerosis
  1. Li Zhenxiao,
  2. Meng Xiaoping
  1. Cardiology, The Second Hospital of Jilin University, China

Abstract

Atherosclerosis is a complex disease, in which oxidative stress in atherosclerosis through the whole process, antioxidant treatment is imperative.

Objective Discuss the effect of N-acetyl cysteine (NAC) in the APOE gene knockout mice in anti-atherosclerosis.

Method In this study, select 8-week-old male Apoe knockout (Apoe-/-) mice were studied, a total of 10, with an average weight of 20 g. Were randomly divided into high-fat diet group (control group), and NAC interfere with treatment group (experimental group), two groups of mice were treated with high-fat diet, NAC intervention group administered a dose of 300 mg/kg, according to body weight of mice in each of the group on the required dose, ministered through drinking water bottle means, and to ensure that set up a daily dose of drug intake. Modelling 60 days, to give a 24-h fasting mice, pick the eye blood, placed in test tubes containing EDTA-specific biochemical tests carried out centrifugation, separated plasma. Off mice were sacrificed, rapid separation of mouse aortic arch and access to paraffin-embedded specimens. By using situ immunofluorescence staining method for the determination of artery intima-mouse levels, to observe the protection of NAC in atherosclerosis during the course of apoptosis; in serological testing, the choice of ELISA method was determined to assess the plasma MMP-9 in mice with ox-LDL levels; artery atherosclerotic plaques in the general morphological study using haematoxylin - eosin (HE) staining method, histological specimens of atherosclerotic lesions in general shape.

Result In the general model of atherosclerosis, the visual plaque morphology: NAC experimental group of mice the distribution of atherosclerotic plaque area less than high-fat diet group; HE stained specimens, NAC group foam cells were markedly reduced plaque in the volume of foam cells and plaque lipid core size significantly decreased compared with the control group; by in situ fluorescence staining method, determination of arterial intima in the expression of MMP-9 positive cells, NAC group of positive cells (6.00±2.45) than the control group (20.08±5.17) (p<0.05), statistically significant; measured the number of apoptotic cells, NAC group number of apoptotic cells (7.80±1.07%) than the control group (9.22±0.84%) (p<0.05), statistically significant; NAC group plasma ox-LDL levels (9.89±1.33 μg/dl) than the control group (15.84±2.60 μg/dl) (p<0.05), NAC group plasma MMP-9 levels (8.50±1.58 ng/ml) than the control group (12.44±2.19 ng/ml) (p<0.05), were statistically significant.

Conclusion NAC can reduce the number of atherosclerotic plaque, plaque distribution area, reducing the number of foam cells, reducing the volume of foam cells in plaques, plaque lipid core volume; NAC can reduce the arterial endometrial expression of MMP-9 in the number of positive cells; NAC can reduce atherosclerotic plaques in the number of apoptotic cells; NAC can reduce plasma ox-LDL and MMP-9 levels. This experiment further confirmed the NAC's anti-atherosclerosis effect of NAC as an anti-atherosclerosis drugs used clinically to provide an experimental basis.

  • N-acetylcysteine
  • atherosclerosis
  • matrix metalloproteinase -9
  • oxidised low-density lipoprotein
  • apoptosis

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