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Basic science: Experiment research
e0216 The expression and role of interleukin-23 in mice virus myocarditis
  1. Fan Yang,
  2. Weifeng Wu,
  3. Yuluan Yan,
  4. Qing Kong,
  5. Yu Pang
  1. The First Affiliated Hospital of Guangxi Medical University, Nanning, China

Abstract

Objective To explore the expression of IL-23 and its role in acute viral myocarditis mice induced by coxsackie virus B3 (CVB3).

Methods To establish the model of VMC, Balb/c male mice were peritoneally injected (IP) with Coxsackievirus B3, mice peritoneally injected with PBS were taken as the controls. On 0, 1, 2, 3, 4 and 6W after IP, haematoxylin-eosin was used to assess pathological changes. The level of IL-23 mRNA in mice myocardic tissue was determined by RT-PCR. The expression of IL-23 protein in blood plasma was evaluated by EILSA.

Results Comparing with the controls, the expression of IL-23 mRNA were steady high from 1W after IP, and maintaining a higher trend until 6W. All the results at different time were higher than those of controls, p<0.05. The expression of IL-23 protein were also higher than those of controls, which in concordance with the changes of mRNA.

Conclusions Our data show that local significantly increased levels of IL- 23p19mRNA in myocardium and IL-23 may play a role in the pathogenesis of mice virus myocarditis.

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