Objective To explore the expression of IL-23 and its role in acute viral myocarditis mice induced by coxsackie virus B3 (CVB3).
Methods To establish the model of VMC, Balb/c male mice were peritoneally injected (IP) with Coxsackievirus B3, mice peritoneally injected with PBS were taken as the controls. On 0, 1, 2, 3, 4 and 6W after IP, haematoxylin-eosin was used to assess pathological changes. The level of IL-23 mRNA in mice myocardic tissue was determined by RT-PCR. The expression of IL-23 protein in blood plasma was evaluated by EILSA.
Results Comparing with the controls, the expression of IL-23 mRNA were steady high from 1W after IP, and maintaining a higher trend until 6W. All the results at different time were higher than those of controls, p<0.05. The expression of IL-23 protein were also higher than those of controls, which in concordance with the changes of mRNA.
Conclusions Our data show that local significantly increased levels of IL- 23p19mRNA in myocardium and IL-23 may play a role in the pathogenesis of mice virus myocarditis.