Objective To study the effect of the Mixture of Shengmai Pulvis and Danshen Decoction in protecting rats of the type 2 diabetic cardiomyopathy (DCM) model.
Methods 42 SD rat models of DCM, established by combination of insulin resistance by a high-fat diet with intraperitoneal injection of high dose streptozotocin (50 mg/kg), were evaluated in the damage of the myocardium by ECG at the twelveth week after modelling, and the serum are analysed for blood glucose (GLU), cholesterol and triglyceride (TG); the content of the left cardiac ventricle myocardial collagen was quantified by Masson staining test; the level of myocardial cell apoptosis was tested with Tunel apoptosis kit; the damage extent of the myocardial subcellular structure was observed by electron microscopy; the expression levels of cardiac TSP-1, TGF-β1 and TRB-3 proteins were detected by immunohistochemistry, the changes of the expression levels of the cardiac TSP-1, A-TGFβ1 and L-TGF-β1 protein were detected by Western blotting; and the changes of the mRNA expression levels of TSP-1 and TRB-3 were detected by real-time quantitative PCR.
Results Compared with the control group, the rat blood glucose, cholesterol, triglycerides were significantly decreased; the myocardial tissue was less damaged and the collagen fibre content was reduced in the group of the Mixture of Shengmai Pulvis and Danshen Decoction; The myocardial sub-cellular structural damage in electron microscopy was to a lesser extent, the expression levels of the myocardial TSP-1, TGF-β1 and TRB-3 by immunohistochemical detection and the average expression levels of the myocardial TSP-1, A-TGFβ1 and L-TGF-β1 by Western blotting were decreased; and the expression levels of TSP-1mRNA and TRB-3 mRNA by PCR detection were decreased than those of the control group.
Conclusion The Mixture of Shengmai Pulvis and Danshen Decoction can inhibit through multiple pathways the process of myocardial fibrosis in the rat myocardium of diabetic cardiomyopathy, and significantly delay the formation course of diabetic cardiomyopathy in hyperglycemia rats.
- The mixture of Shengmai Pulvis and Danshen Decoction
- diabetic cardiomyopathy
- collagen fibre