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Basic science: Experiment research
e0220 The involvement of IL-23/Th17 pathway in murin model of coxsackie virus B3-induced viral myocarditis
  1. Fan Yang,
  2. Weifeng Wu,
  3. Yuluan Yan,
  4. Qing Kong,
  5. Yu Pang
  1. Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China

Abstract

Background The IL-23/Th17 pathway plays an important role in the development of chronic inflammatory diseases and autoimmune diseases. However, the role of the IL-23/Th17 axis in the regulation of virus myocarditis (VMC) is still largely unknown.

Methods VMC was induced in male Balb/c mice by CVB3 peritoneal injection. Mice injected with PBS were taken as the controls. IL-23, IL-17 and RORγt mRNA in the myocardium of VMC were assessed by semi-quantitative RT-PCR on the time of 0, 1, 2, 3, 4 and 6 weeks after injection. IL-23, IL-17 protein from blood plasma was evaluated by ELISA. Flow cytometric analysis was used to evaluate the frequencies of Th17 subsets in CD4+Tcell. CD4+ T cells were isolated from VMC mice and cultured with rIL-23 in vitro to investigate the function of IL-23 in the IL-23/Th-17 pathway.

Results Comparing with the controls, IL-23, IL-17 and RORγt mRNA were steadily expressed in the myocardium of infected mice from 1 week after virus injection (p<0.01), IL-23 and IL17 protein level increased from 1st week to 6th week. The frequencies of Th17 cells were obviously increased in VMC mice 1 week after infection (p<0.01), the maximum level of Th17 cells was reached at 4th week. The ratio of Th17 cells in the spleen lymphocyte significantly improved after rIL-23 stimulation, the IL-17 and RORγt mRNA expression of the cultured cells and the IL-17 protein in the culture supernatants increased after rIL-23 stimulation (p<0.05).

Conclusions IL-23/Th-17 pathway may play an essential role in VMC.

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