Objective To observe the effects of extracorporeal cardiac shock wave therapy (CSWT) on cintent of endothelial nitric oxide synthase (eNOS) and basic fibroblast growth factor (bFGF) in serum and myocardial tissue in swine with acute myocardial infarction (AMI).
Methods 12 Model swines with acute myocardial infarction was made, and were randomly divided into two groups with six in each group: simplex myocardial infarction as the control group and the experimental group which received CSWT treatment. (three times on the first, third, fifth days after operation. 200 hit/point. Total: 12 points. Energy: 0.09 mJ/mm2). Peripheral blood was extracted at eight different time points before and after operation (immediate, the first, third, fifth days, 1, 2, 3, 4 weeks after operation) to detect serum eNOS content with enzymelinked immunosorbent assay. Materials at myocardial tissue from the swines killed 1 month later were obtained to detect expression amount of eNOS and bFGF with semi quantitative RT-PCR method.
Results eNOS rose up in the experimental group 1 day after CSWT and a reached the peak on the fifth day, whereas eNOS gradully reduced in the control group, which slowed significant difference between the two groups (p<0.01). Detection with semi quantities RT-PCR of myocardial tissues of infarction border area showed that eNOS and bFGF expression in the experimental group were obviously higher than those in the control group ((eNOS 27.705±4.13) vs (16.448±3.21) (bFGF 32.571±4.23) vs (17.858±4.17 p<0.01)).
Conclusions Extracorporeal cardiac shock wave therapy of acute myocardial infarction can effectively promote rise of eNOS and bFGF, which may be a new way to cure AMI.
- Extracorporeal cardiac shock wave therapy
- acute myocardial infarction
- endothelial nitric oxide synthase
- basic fibroblast growth factor