Objective To evaluate the transfection efficiency of recombinant adeno- associated virus serotype 9 carrying enhanced green fluorescent protein (rAAV9- eGFP) to mouse heart in vivo and the effects on cardiac function.
Methods 1. 16 C57BL/6 mice were transfected rAAV9-eGFP by tail injection. EGFP expression in the heart, liver, lung, kidney and brain cryosections was observed under inverted fluorescence microscope 7, 14, 21, 28 days after the injection of rAAV9-eGFP and eGFP was quantitated by Western Blot. 2. 20 C57BL/6 mice were divided into control group and rAAV9-eGFP group randomly, and were received with saline or rAAV9-eGFP. The echocardiography and haemodynamics were performed 28 days after the injection of saline or rAAV9-EGFP.
Results 1. EGFP expression in the heart reached the maximum at day 21, at the point of which the transduction efficiency of rAAV9-eGFP in myocardium was 32%. The other tissues had a little or no eGFP expression. 2. The cardiac function did not reveal significant difference between rAAV9-eGFP group and the control group after transfection (p>0.05).
Conclusion rAAV9-eGFP gene can be stably and efficiently expressed in mouse heart, and has no toxic effect on cardiac function.