VSMCs Migration and proliferation are the critical pathological processes of various cardiovascular disease, such as atherosclerosis. Luteolin is a kind of flavonoids naturally occurring in many vegetables, fruits and medical plant. In this study, we investigated the effect of luteolin on the proliferation and migration of rat vascular smooth muscle cells (VSMCs) which were stimulated by H2O2, and a primary discussion was given to its mechanism of action. The present study demonstrated that Luteolin (12.5 to 50 μM) showed a particularly concentration-dependent inhibition effect on H2O2-elicited VSMCs proliferation and migration by MTT and Transwell assay respectively. In further research, we originally explored the function of Luteolin in blocking H2O2-triggered Src and Akt signalling pathways. The activation of Src, PDK1, Akt (308), Akt (473) of Luteolin group was significant lower than that of H2O2 group. These findings strongly suggested that Luteolin suppressed H2O2-directed migration and proliferation in VSMCs by inactivating Src and Akt pathways which participate in VSMCs migration and proliferation.