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Basic science: Experiment research
e0236 A reperfusion model in AMI rabbits
  1. Zhang Jun1,
  2. Qi Xiao-yong2,
  3. Wan Yan-fang1,
  4. Yuan Chen1,
  5. Ma Zhan-feng1,
  6. Wang Ning1,
  7. Dang Yi2,
  8. Yuan Bo-min1
  1. 1Department of Cardiology, Cangzhou Central Hospital, Affiliated Hospital of Hebei Medical University, Cangzhou, China
  2. 2Department of Cardiology, Hebei General Hospital, China

Abstract

Objective To Explore the feasibility of establishing the reperfusion model on AMI rabbit by the method of obstructing and releasing the Left Anterior Ventricular Branch (LAVB) of left circumflex coronary artery (LCX).

Methods A total of 24 healthy Japanese albino rabbits of both sex were used in this study. Rabbits were randomly divided into 3 groups: ischaemia-reperfusion group (IR group, 8), ischaemia-noperfusion group (AMI group, 8), sham group (sham, 8). After preconditioning the myocardium twice by obstructing the blood flow for 5 min, we obstructed the flow of LAVB in IR group for 60 min, and then released it to be reperfusion. In AMI group we obstructed the flow permanently by ligating LAVB. And in sham group we only threaded but did not obstruct the flow. Then we killed them 3 days later. Venous blood was gathered. The levels of cTNI, CK and CK-MB were assayed at pre-reperfusion (baseline) and post-reperfusion period (4-h, 8-h, 12-h, 24-h, 48-h and 72-h after being reperfusion). The summation changes of ST segment elevation were observed in leads П, Ш, avF by ECG. Histopathology of myocardia, Evan's Blue and TTC dyeing were taken into notice. STATA8.0 software pack was used for data analysis.

Results The results come out that the ST segment all elevated in each group after LAVB was obstructed. In IR group the ST segment lowered more than 50% within 120 min after releasing the artery. This phenomenon had not appeared in other two groups. Compared to baseline, the cTNI, CK and CK-MB were all raised in IR group, and the peak value antedisplaced to 8 h, 12 h and 10 h. These three factors were all raised but no antedisplacement of the enzyme peak in AMI group. In sham group the raising of the three factors was slight, and no antedisplacement of the enzyme peak either. The experiment of rabbits in IR and AMI groups were consistent with the AMI diagnostic criteria in AMI Guideline of diagnosis and therapy established by Cardiac Disease branch of Chinese Medical Association in 2004. The histopathology examination and TTC staining agree with the AMI diagnosis too. The experiment of rabbits in IR group was in accordance with the recanalisation criteria of IRCA recommended by the Chinese J Cardiol editorial committee in 1991. The injection of Evan's Blue was in coincidence with the recanalisation of IRCA too.

Conclusions AMI-reperfusion rabbit models can be successfully established by the application of this method. It has proved to be very effective.

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