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Basic science: Experiment research
e0240 Effects of atorvastatin on the expressions of PECAM-1 and P-selectin in atherosclerotic rabbits
  1. Liao Wei,
  2. Cai Jiumei,
  3. Li Faquan
  1. The Affiliated Hospital of Gannan Medical College, Jiangxi, China

Abstract

Objective To investigate the effects of atorvastatin on atherosclerotic rabbits serum lipid concentration and the expressions of PECAM-1 and P-selectin in rabbit thoracic aorta.

Methods Atherosclerotic rabbit models were made by feeding the rabbits with high cholesterol. Thirty New Zealand big rabbits, weight (2.0±0.2) kg, were randomly divided into three groups. Group A was fed with common forage, Group B was fed with high cholesterol diet, and group C was fed with high cholesterol diet and atorvastatin simultaneously. After 16 weeks feeding, serum TC, TG, LDL-C, VLDL-C were measured. The aortic arteries were isolated and the samples adjacent to aortic arch were harvested for formicroscopy detection. The PECAM-1 and P-selectin gene expressions in rabbit thoracic aorta were detected by RT-PCR.

Results The levels of TC, TG, LDL-C, VLDL-C in group B were significantly increased compared with group B and group C (p<0.05), while the levels of TC, TG, LDL-C, VLDL-C in group C were higher than those in group A (p<0.05). The aorta structure in group A was clear, the aortic intimal in group B was significantly thick and infiltrated by a large number of foam and inflammatory cells, but the endothelium in group C only showed focal infiltration of those cells observed under the microscope. The mRNA expression of PECAM-1 and P-selectin in group B were significantly increased than in group A (p<0.01), while those in group C, which were also higher than group A, were significantly decreased than in group B (p<0.05).

Conclusions Atorvastatin regulates lipid metabolism effectively and reduces the PECAM-1 and P-selectin expression, which are closely related to atherosclerosis.

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