Objective Ischaemic heart diseases, such as acute myocardial infarction, is the most important reason of heart failure. It has been thought that adult cardiac muscle cell is terminal differentiation and would not be regenerated. So now in the clinical, the myocardial ischaemia and other complications cannot be treated thoroughly. Stem cells have got a lot of attention because of the ability of self-renewal and multi-directional differentiation. Compared with other stem cells, an important clinical advantage of adipose tissue derived stem cells (ADSCs) is that they can be isolated in real time in sufficient quantity with small injuries, so the adipose tissue will have a potential perspective. The objective of this research is to discuss the therapeutical effect of the ADSCs on acute myocardial infarction.
Methods Ligated the left anterior descending coronary artery of the SD rats to make a model of acute myocardial infarction. The transplantated cells were isolated from the groin and the scapular adipose tissue of the eGFP mice. The ADSCs were transplanted into the infarction area of the experimental group (n=8) by injection but PBS with the same volume as the control group (n=8). Four weeks later, the heart function was measured with echocardiography. The infarction size and the thickness of the ventricular wall were measured by using Masson trichrome stain. All data were expressed as mean±SEM. Differences between groups were analysed for statistical significance by the independent sample t-test using SPSS Statistics 11.5. A p-value less than 0.05 denoted a statistically significant difference.
Results Compared with the control group, there were smaller infarction size (40.0±8.7% vs 79.3±27.1%, p<0.01), thicker wall (564.1±148.1 μm vs 189.7±70.0 μm, p<0.01) in the experimental group as well as the well improved echocardiographic functional parameters, LVESD: (0.34±0.17 cm vs 0.49±0.25 cm, p<0.01), LVEDD: (0.50±0.19 cm vs 0.61±0.27 cm, p<0.05), LVFS: [(31.5±1.8)% vs (19.4±1.4)%, p<0.01], LVEF: [(65.6±3.6)% vs (45.3±2.8)%, p<0.01].
Conclusion By injecting into the infarct cardiac muscle, the ADSCs have a positive effect to improve the reconstruction and the systolic and diastolic function in a rat model of acute myocardial infarction.