Purpose To investigate the changes of EPCs proliferation and related factors in peripheral blood after the CSWT treatment.
Methods A total of 26 CAD patients undergoing extracorporeal cardiac shock wave therapy were selected. Mononuclear cells obtained from peripheral blood were cultured in EGM-2-MV medium. Cell morphology and the number of colonies formed were observed and analysed. After 7 days of culture, adherent cells were analysed and counted. Whether EPCs differentiated or not was identified by laser confocal microscopy; the number of circulating EPCs were studied by flow cytometry; the plasma level of VEGF, IL-8, SDF-1 and MMP-9 was determined by enzyme linked immunosorbent assay.
Results The cultured EPCs and EPC-CFU number in vitro were significantly increase after therapy (EPCs (18.85±4.30) cell/high power field vs (30.12±6.77) cell/high power field (5.08±1.79) cell/high power field vs (12.27±2.75) cell/high power field, all p<0.001). Circulating EPCs number were significantly increased ((0.015±0.003)% vs (0.021±0.005)%, p<0.001), VEGF, IL-8 level were significantly increased (VEGF (120.26±19.85) pg/ml vs (155.19±24.67) pg/ml, IL-8 (21.81±5.94) pg/ml vs (149.70±44.11) pg/ml, all p<0.01), whereas SDF-1 and MMP-9 had no significant changes (SDF-1 (2750.87±636.74) pg/ml vs (2700.47±415.19) pg/ml, MMP-9 (19.66±3.96) ng/ml vs (18.55±3.78) ng/ml, all p>0.05), compared with the group before treatment.
Conclusions The CSWT appears to promote the expression of VEGF and IL-8 proteins, also stimulates the EPCs proliferation, significantly increases the EPCs and improves its function in peripheral blood, whereas the CSWT likes not influence so obviously on the expression of SDF-1, MMP-9.