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Experimental research
High density lipoprotein induces rats mesenchymal stem cells proliferation through activating PI3K-Akt pathway
  1. Jianfeng Xu1,
  2. Juying Qian1,
  3. Xinxing Xie2,
  4. Jianying Ma1,
  5. Li Lin2,
  6. Mingqiang Fu1,
  7. Aijun Sun1,
  8. Yunzeng Zou1,
  9. Junbo Ge1
  1. 1Zhongshan Hospital, Shanghai, China
  2. 2Eastern Hospital, Hong kong, China

Abstract

Objective To explore the effect of high density lipoprotein (HDL) on the proliferation of mesenchymal stem cells (MSCs), and to elucidate the role of PI3K-Akt pathway in the potential regulation of it.

Methods MSCs were collected from the femora of Sprague–Dawley rats and were treated with HDL in different concentration (0, 20 ug/ml, 50 ug/ml, 100 ug/ml) for 24 h; and then were treated with HDL (50 ug/ml) for 24 h, 48 h and 72 h, respectively. The proliferation of MSCs in each group was compared by Cell Counting Kit-8 (CCK-8) and BrdU cell proliferation assay. The expression of phosphorylation of Akt was evaluated by Western Blotting. LY294002, an inhibitor of PI3K, was used to down-regulate the activity of PI3K-Akt pathway.

Results The results showed that HDL induces markedly MSCs proliferation in time- and concentration-dependent manner. Akt phosphorylation was significantly increased by 2.35-, 4.52-, and 5.89-folds after simulation by 20 ug/ml, 50 ug/ml and 100 ug/ml HDL for 24 h (p value all<0.05). And when incubated with HLD (50 ug/ml), the phosphorylation of Akt was activated at 15 min, and peaked at 60 min With the use of LY294002, the proliferation of MSCs was attenuated by 32% (26∼40%, p value<0.05) when treated with HLD (50 ug/ml) for 24 h.

Conclusion HDL improved the proliferation of MSCs in time- and concentration-dependant manner, and PI3K/Akt pathway was one of the underlying mechanisms involved in it.

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