Article Text

PDF

Cardiovascular disease basic research
The study on human umbilical vein endothelial cells (HUVECs) proliferation and the best energy in shock waves treatment
  1. Hongyan Cai1,
  2. Tao Guo2,
  3. Yu Wang3,
  4. Lin Li2,
  5. Lin Zhao3,
  6. Xiyun Yang2
  1. 1Department of Cardiology, The First Affiliated Hospital Kunming Medical College
  2. 2The First Affiliated Hospital
  3. 3Kunming Medical College

Abstract

Purpose To explore how different intensity of ultrasonic shock energy influences the human umbilical vein endothelial cells (HUVECs) proliferation, differentiation and the related cytokines, and to ascertain the best energy of CSWT.

Methods The HUVECs cell lines were performed using different levels of energy (0, 0.03, 0.09, 0.18, 0.24 mJ/mm2) shock wave treatment in vitro, Utilising CCK8 colorimetric method to detect HUVECs proliferation, real time PCR, Western blotting and Flow Cytometry were utilised to detect the changes in mRNA and protein of VEGF, IL-8, ICAM-1 before and after CSWT treatment.

Results The 0.09 mJ/mm2 shock energy significantly promoted the HUVECs proliferation (p<0.05). The results from real time PCR revealed that 0.09 mJ/mm2 treatment markedly increased the expression of VEGF, IL-8, ICAM-1 (p<0.001, respectively) compared with the non-treated control, the expression of ICAM-1 0.03 mJ/mm2 group were increased compared with the control group (p<0.01) While the expression of VEGF, IL-8 showed no significant changes (p>0.05, respectively), 0.18 mJ/mm2 treatment markedly increased the expression of VEGF (p<0.001) compared with the non-treated control While the expression of ICAM-1, IL-8 showed no significant changes (p>0.05, respectively), 0.24 mJ/mm2 treatment showed no significant increase in the expression of VEGF, IL-8, ICAM-1(p>0.05, respectively) compared with the non-treated control. Western blot analysis and Flow Cytometry showed that the expression of VEGF, IL-8, ICAM-1 in 0.09 mJ/mm2 group were significantly higher than the control group (p<0.05, respectively); the expression of VEGF in 0.03 mJ/mm2 group was significantly higher than the control group (p<0.05) While the expression of ICAM-1, IL-8 was not significantly higher than the control group p>0.05, respectively); the expression of VEGF in 0.18, 0.24 mJ/mm2 group was significantly higher than the control group (p<0.05) While the expression of ICAM-1, IL-8 was not significantly higher than the control group (p>0.05, respectively).

Conclusions (1) The different extracorporeal shock wave energy promoted the HUVECs proliferations to different extent, the effect of 0.09 mJ/mm2 energy is the most evident. (2) The 0.09 mJ/mm2 energy increase the expression of IL-8, ICAM-1 mRNA and protein most significantly. (3) The 0.09 mJ/mm2 energy increased the expression of VEGF mRNA and protein most remarkably. (4) The 0.03∼0.24 mJ/mm2 energies also have some effects in facilitating the secretion of VEGF, IL-8 and ICAM-1.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.