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Cardiovascular disease basic research
Correlation of the pregnancy-associated plasma protein-A gene IVS6+95 polymorphism with the serum PAPP-A level in patients with acute myocardial infarction
  1. Guoping He,
  2. Fengjie Liu
  1. Department of Cardiology, Affiliated Wujin Hospital of Jiangsu University, Changzhou, China

Abstract

Objective To investigate the association between PAPP-A gene IVS6+95 polymorphism and the serum level of PAPP-A in patients with AMI from the Chinese Han population of Sunan region.

Methods Fifty-six patients with AMI (AMI group) and 51 control subjects who were free from coronary artery disease confirmed by coronary angiography (control group) were recruited into the study. The PAPP-A gene IVS6+95 polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP). The serum level of PAPP-A were determined by enzyme-linked immunosorbent assay (ELISA).

Results As compared with those in the control group, the frequencies of the CC genotype in AMI group was higher (11.8% vs 28.6%, p=0.032) and the frequencies of the CG (41.2% vs 32.1%) and GG (47.1% vs 39.3%) genotype, and C allele (35.3% vs 48.2%) was not different (all p value>0.05). Multivariate logistic regression analysis showed that there was significant correlation of the CC genotype (OR=3.382, 95% CI 2.798 to 3.966, p=0.037) and C allele (OR=2.093, 95% CI 1.753 to 2.443, p=0.030) with the risk of AMI, The serum level of PAPP-A (M/IQR) was significantly higher in AMI group (8.59/148.91 pg/ml) than that in the control group (0.05/14.42 pg/ml) (p<0.001). No significant differences were found in the serum level of PAPP-A among any of the genotypes of the PAPP-A gene IVS6+95 within AMI group and its subgroup (the same gender group and the same age group), and control group, respectively (all p value>0.05), suggesting no association of the PAPP-A gene IVS6+95 polymorphism with the serum level of PAPP-A. Multiple linear regression analysis showed that the serum level of PAPP-A was not associated with CHD-associated factors such as age, gender, smoking, history of diabetes mellitus and hypertension, and dislipidemia in AMI group. Conclusion: In the Chinese Han population of Sunan region, there is the IVS6+95 polymorphism of PAPP-A gene, which may be associated with attack of AMI but not associated with the serum level of PAPP-A; the serum PAPP-A level is significantly increased and not affected by CAD relevant factors in patients with AMI, which suggest that there is an association of the serum level of PAPP-A with unstable plaque and may be an independent risk factor for attack of AMI.

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