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Cardiovascular disease basic research
Association of ALOX5AP gene SG13S114T/A polymorphism with acute coronary syndrome
  1. HE Guoping,
  2. HUI Jingjiao,
  3. SHEN Dandan
  1. Department of Cardiology, Affiliated Wujin Hospital of Jiangsu University, Changzhou, China

Abstract

Objective To investigate the distribution of ALOX5AP gene SG13S114T/A polymorphism and the association of the ALOX5AP gene SG13S114T/A polymorphism with acute coronary syndrome (ACS) in the Chinese Han population of Sunan region.

Methods This study was conducted with a case-control design including 545 patients with ACS (ACS group) and 567 control subjects who were free from coronary artery disease (control group). ALOX5AP gene SG13S114T/A polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism analysis.

Results There were AA, AT and TT genotypes of the ALOX5AP gene SG13S114T/A polymorphism both in ACS group and control group. The genotype distribution of the ACS group and control group conformed to the Hardy-Weinberg balance via χ2 test (p>0.05), which suggested that the selected sample was representative. As compared with those in the control group, the genotype frequency of AT (37.57% vs 48.99%, p=0.015) was higher, the genotype frequency of TT (48.15% vs 38.17%, p=0.034) was lower, and the frequencies of AA genotype (14.28% vs 12.84%, p=0.054) and T allele (66.93% vs 62.66%, p=0.330) were not significantly different (all p>0.05) in ACS group. Subgroup analysis showed that as compared with those in control group respectively: (1) the genotype frequency of AT (30.30% vs 56.41%, p=0.003) was higher, the genotype frequency of TT (53.30% vs 32.50%, p=0.017) was lower, and the frequencies of AA genotype (16.67% vs 13.54%, p=0.263) and T allele (68.33% vs 60.26%, p=0.331) were not significantly different (all p>0.05) in AMI group; (2) the genotype frequency of AT (30.30% vs 47.14%, p=0.045) was higher, the frequencies of AA (16.67% vs 10.00%, p=0.146) and TT (53.33% vs 42.86%, p=0.291) genotypes and T allele (68.33% vs 60.26%, p=0.727) were not significantly different (all p>0.05) in UAP group; (3) the frequency of T allele (68.06% vs 82.42%, p=0.046) was higher in male ACS group and not significantly different (p>0.05) in female ACS group, there were no significant statistical difference of the genotype frequencies of AA ((13.19% vs 8.02%) and (5.41% vs 14.04%)), AT ((37.50% vs 33.69%) and (37.99% vs 45.03%)) and TT ((49.31% vs 58.29%) and (46.59% vs 40.94%)) in male ACS group and female ACS group (all p value >0.05); (4) the genotype frequency of AT (38.58% vs 50.93%, p=0.041) was higher, the genotype frequency of TT (48.66% vs 35.28%, p=0.020) was lower, the frequencies of AA (12.76% vs 13.79%, p=0.722) genotype and T (61.22% vs 60.74%, p=0.931) allele were not significantly different in elderly ACS group; (5) the frequencies of AA (16.96% vs 10.11%, p=0.127), AT (36.09% vs 50.00%, p=0.078) and TT (46.96% vs 39.39%, (p=0.338) genotypes and T allele (65.00% vs 64.29%, p=0.932) were not significantly different in premature ACS group. Multivariate logistic regression analysis showed that there was statistically significant correlation of AT and TT genotype, and T allele with ACS (P was 0.001, 0.001 and 0.031, respectively). Furthermore, subgroups analysis showed that AT and TT genotype were correlated with AMI (all p<0.001); AT genotype was correlated with UAP (p=0.007); AT and TT genotypes, and T allele were correlated with male ACS (p<0.001, was 0.001 and 0.016, respectively); AT and TT genotypes, and T allele were correlated with the elderly ACS (p was 0.004, 0.001 and 0.013, respectively).

Conclusion Three genotypes including AA, AT and TT genotypes exist in the ALOX5AP gene SG13S114T/A both in ACS group including its subgroups and control group. There was statistically significant association of the SG13S114T/A polymorphism of ALOX5AP gene with risk of ACS, AMI, UAP, male ACS and the elderly ACS in the Chinese Han population of Sunan region, which suggest ALOX5AP gene SG13S114T/A polymorphism play a potential role in the origin and development of ACS.

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