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Cardiovascular disease basic research
Molecular mechanisms of ionic remodeling in L-type voltage dependent calcium channel of age-related change in atrial of canine
  1. Zhou Xianhui,
  2. Gan Tianyi,
  3. Xu Guojun,
  4. Tang Baopeng,
  5. Mao Ting,
  6. Zhang Jian
  1. The First Affiliated Hospital Of Xinjiang Medical University, Urumqi, Xinjiang, China

Abstract

Purpose The present study aims to investigate the molecular basis of L-type voltage dependent calcium channel (LVDCC) in adult and aged canine.

Methods The action potential duration (APD90), amplitude of action potential plateau, ICa-L peak current density were of LVDCC, recorded by patch clamp technique. The mRNA gene and protein expression levels of α1c subunit (CaV1.2), sarcoplasmic reticulum Ca2+-ATPase (SECRA2), Calpain-I, ryanodine receptor (RyR2) were detected by semi-quantitative RT-PCR.

Results ICa-L peak current density was (−14.04±0.82 pA/pF), in adult group compared with (−8.11±0.54 pA/pF, p<0.05) in the aged group and action potential duration to 90% repolarisation (APD90) of aged group was significantly decreased. The mRNA gene expression levels of CaV1.2 was significantly lower in the aged dogs (0.9±0.35) than in the adult dogs (2.38±0.4, p<0.05), The mRNA gene expression levels of RYR2 was significantly higher in the aged dogs (4.39±4.68) than in the adult dogs (1.49±1.69, p<0.05). There were not significantly different gene expression levels of SECRA2 and calpain I in two groups; The protein expression levels of CaV1.2 were significantly lower in the aged dogs than in the adult dogs (0.13±0.10 vs 0.29±0.12, p<0.05), The protein expression levels of RYR2 was significantly higher in the aged dogs than in the adult dogs (0.18±0.21 vs 0.08±0.36, p<0.05), There were not significantly different protein expression levels of SECRA2 and Calpain I in two groups.

Conclusion These data suggest that the change of mRNA and protein expression of CaV1.2 and RYR2 of LVDCC may serve as the molecular basis of ICa-L remodeling respectively in aged dogs. This plays an important role in the predisposition to developing atrial fibrillation (AF) due to ageing.

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