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Cardiovascular disease basic research
The evaluation of the effect of hydrogen sulfide postconditioning by P-V loop against myocardial I/R injury in rats
  1. Chenghai Peng1,
  2. Sa Shi2,
  3. Zhitao Li2,
  4. Yanjun Chen1,
  5. Xueqi Li1,
  6. Ye Tian3
  1. 1Department Of Cardiology, The Forth Affiliated Hospital, Harbin Medical University, Harbin, China
  2. 2Department Of Pathophysiology, Harbin Medical University, Harbin, China
  3. 3Department Of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, China

Abstract

Objective To evaluate the effect of hydrogen sulfide (H2S) postconditioning on myocardial ischemia-reperfusion (I/R) by pressure-volume loop (P-V loop).

Methods The I/R model of rat in vivo was established by ligating the left anterior descending coronary artery for 30 min and reperfusing for 120 min. Wistar rats (n=32) were randomly divided into four groups: Sham operation, ischemia-reperfusion (I/R), Ischemic postconditioning (IPO) and H2S postconditioning. In sham operation, there was no ligation. In IPO, at the start of reperfusion, three cycles of 30 s reperfusion and 30 s LAD reocclusion preceded the 3 h of reperfusion. In H2S postconditioning, NaHS (15 μmol/kg, Sodium hydrosulfide) was administrated before coronary artery reperfusion. The heart rate (HR), I/R arrhythmia, the left ventricular end-systolic pressure (LVESP), left ventricular end-diastolic pressure (LVEDP), the slope of the end- systolic P-V relation (ESPVR) and the slope of the end-diastolic P-V relation (EDPVR) were detected. Infarct size was determined by scanning the images of the rat heart ventricular sections stained with Evans blue and TTC.

Results Compared with I/R and IPO group, the I/R arrhythmia and the infarct size were decreased significantly (p<0.05, p<0.05). The parameters of heart function evaluated by P-V loop were improved greatly (p<0.01) in H2S postconditioning group compared to that in I/R group, but there was no significant difference between H2S postconditioning and IPO group.

Conclusion Myocardial I/R injury was decreased by H2S postconditioning, and it was sensitive and accurate to evaluate the heart function by P-V loop.

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