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Cardiovascular disease basic research
Autoantibodies against β3-adrenoceptor reduce the susceptibility to myocardial ischemic/reperfusion injury of heart failure rats
  1. Ma Xiurui1,
  2. Zhang Lizhen1,
  3. Wang Jingping1,
  4. Guo Li1,
  5. Zhang Zhulin1,
  6. Liu Huirong2
  1. 1Shanxi Cadiovascular Hospital, Shanxi, China
  2. 2Capital Medical University, Beijing, China

Abstract

Objective Choosing chronic heart failure rats as starting point, to observe whether Autoantibodies against β3-adrenoceptor (β3AA) can reduce the susceptibility to myocardial ischemic/reperfusion injury of heart failure rats, then to study whether β3AA have cardio protective effect to the heart failure rats.

Methods β3AA was produced through actively immunised method; The level of β3AA in the sera was detected by ELISA, β3AA positive/negative IgG were purified by Mab Trap Kit (Amersham, USA), BCA Protein Assay kit (Pierce, USA) was used for protein quantitation, the purities of extractions were assessed by conventional SDS-PAGE; Aortic banding surgery was used to prepare the heart failure model, Ligating the left anterior descending coronary artery for 30 min, then loose the ligature for reperfusion 3 h to make the myocardial ischemia/reperfusion injury, TTC coloration is used to detect the myocardial infarct sise, caspase 3 ability assay and TdT-mediated dUTP nick end labelling were used to detect the apoptosis.

Results After β3AA was administrated to the rats, the myocardial infarct size induced by ischemia/reperfusion injury was smaller than that in the control group (48.81±6.45% vs 60.25±2.52%, p<0.05); after the heart failure rats undergo the myocardial ischemia/reperfusion injury, the TUNEL-positive cells and the apoptosis index both were significantly lower in β3AA positive group than in β3AA negative group (24.33±1.82% vs 31.24±1.31%, p<0.01); moreover compared with the β3AA negative group, the caspase-3 activity in myocardiocytes was also lower in β3AA positive group (1941±150.0 vs 1485±89.39, p<0.01).

Conclusion β3AA can decrease the myocardial infarct size and cardiomyocyte apoptosis induced by ischemia/reperfusion injury in heart failure rats, so β3AA reduce the susceptibility to myocardial ischemic/reperfusion injury of heart failure rats.

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