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Cardiovascular disease basic research
The combination therapy of cilostazol and ischemic postconditioning protects against ischemia-reperfusion injury in non-diabetic and diabetic rat hearts with long-term atorvastatin treatment
  1. Fan ying,
  2. Yang shusen,
  3. Cao yang,
  4. Huang yonglin,
  5. Tian ye
  1. The First Hospital of Harbin Medical University, Harbin, China

Abstract

Objective This study is to investigate if the combination therapy of cilostazol and ischemic postconditioning (Ipost) could protect the non-diabetic and diabetic rat hearts against reperfusion injury after long-term atorvastatin treatment.

Methods Non-diabetic and diabetic rats were randomly assigned to six groups: (1) nonconditioning, (2) Ipost, (3) atorvastatin (2 mg/kg/day for two weeks), (4) atorvastatin and Ipost, (5) atorvastatin and cilostazol (20 mg/kg cilostazol once before reperfusion), (6) atorvastatin, cilostazol, and Ipost. Infarct sise, haemodynamics and expression of Akt and eNOS were examined.

Results Long-term atorvastatin treatment without or with Ipost didn't decrease reperfusion injury in non-diabetic and diabetic rat hearts. However, the cardioprotective effects were shown in the group of statin, cilostazol and Ipost (25.0±4.9% and 31.1±5.5% in non-diabetic and diabetic hearts), but not in the group of statin and cilostazol (44.8±4.76% and 57.7±5.2%). Western blot results revealed that Akt and eNOS phosphorylation were detected in the combination treatment of cilostazol and Ipost after long-term statin treatment.

Conclusions The combination therapy of cilostazol and Ipost could reduce reperfusion injury via increasing Akt and eNOS phosphorylation in statin-treated non-diabetic and diabetic hearts.

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