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Cardiovascular disease basic research
Anti-apoptosis effects of Captopril, Milkvetch root and their combination on HUVEC induced by H2O2
  1. Zhang Jing,
  2. He Shenghu
  1. Jiangsu Province Subei Renmin Hospital, Jiangsu, China

Abstract

Objective To investigate the protective effects and mechanisms of ACEI (Captopril), Milkvetch root and their combination on human umbilical vascular endothelial cells (HUVECs) following the damage of hydrogen peroxide (H2O2).

Methods HUVEC were injured for 4 h by 2.00 mmol/l H2O2 after they were incubated with different dosages of Captopril, Milkvetch root and their combination for 24 h. AO/EB fluorescent staining observed morphology changing of apoptosis. Apoptosis ratio of HUVEC was detected by FCM. Important inhibition apoptosis proteinum Bcl-2 was detected by Western blot.

Results The morning apoptosis ratio of cell in the normal control and (Captopril-L, M, H/Milkvetch root-M, H)+H2O2 groups were significantly lower than those in the H2O2 oxidative control (p<0.05 or p<0.01) except Milkvetch root-L+H2O2 group (p>0.05). The total apoptosis ratio of cell in the normal control and (Captopril/Milkvetch root-M, H)+H2O2 groups were significantly lower than those in the H2O2 oxidative control (p<0.05 or p<0.01) except Captopril/Milkvetch root-L+H2O2 group (p>0.05). Besides the content of important inhibition apoptosis proteinum Bcl-2 of cell in the normal control and (Captopril/Milkvetch root-M, H)+ H2O2 groups were significantly lower than those in the H2O2 oxidative control (p<0.05 or p<0.01) except (Captopril/Milkvetch root -L)+H2O2 group (p>0.05). The effect of (Captopril+Milkvetch root)-L, M, H+H2O2 groups were significantly better than those in the Captopril/Milkvetch root-L, M, H+H2O2 groups (p<0.05 or p<0.01). Conclusion Captopril, Milkvetch root and their combination can decrease in the morning and apoptosis ratio induced by H2O2, increases the level of proteinum Bcl-2 of cell, and depends on their concentration to some extent. The combined effect of Captopril and Milkvetch root is better than the single effect of Captopril and Milkvetch root. So the combination of Medicine and Chinese traditional medicine can better protect damaged HUVECs.

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