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Experimental research
Vitamin C promotes differentiation of mouse induced pluripotent stem cells into cardiomyocyte in vitro
  1. Yang Liang,
  2. Zhang Xiao-Gang,
  3. Wei Xin-Wei
  1. Department of Cardiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China

Abstract

Objective To investigate the effect of Vitamin C on the cardiomyocytes differentiation of mouse induced pluripotent stem cells (miPS).

Methods Embryoid bodies (EBs) formed from miPS were induced by differentiation medium containing various concentration of Vitamin C.

Results The best concentration of Vitamin C to promote the cardiac differentiation of miPS is 10−4 mol/l, beating area was found in 57.00%±3.20% of EBs. There was a significant difference compared with the control group 5.13%±0.55% (p<0.01). Special cardiac proteins α-actin and cTnT were detected.

Conclusion Vitamin C could redound differentiation of miPS to cardiomyocytes, and it is a safe and a highly efficient method in vitro.

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