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GW23-e1066
EFFECT OF CELL-CELL DIRECT CONTACT TO MESENCHYMAL STEM CELLS DIFFERENTIATE INTO VASCULAR ENDOTHELIAL CELLS
  1. Jing Peng,
  2. Luxiang Chi
  1. Department of Cardiology, Southwest Hospital, Third Military Medical University, Chongqing

    Abstract

    Objectives To investigate the effect of cell-cell direct contact to mesenchymal stem cells (MSCs) differentiate into vascular endothelial cells (VECs) when MSCs co-cultured with human umbilical vein endothelial cells (HUVECs) in vitro.

    Methods MSCs and HUVECs were cultured and identified, and then co-culture. The VEGF levels of experimental group (culture media of co-cultured for 48 h) and control group (MSCs and HUVECs's mixed culture media after them were cultured alone for 48 h) were confirmed using ELISA assays. Co-cultured for 5 days, The MSCs's expression of fetal liver kinase-1 (Flk-1) and von Willebrand factor (vWF) and ingestible ability of Dil-ac-LDL was analysed by immunofluorescence staining, and the changes of ultrastructure of co-cultured cells were observed.

    Results The VEGF levels of experimental group was significantly higher than control group; In direct contact co-culture system, some MSCs were induced to express Flk-1 protein, and part of the Flk-1 positive MSCs also expressed vWF protein ;some MSCs could phagocytose Dil-ac-LDL; the reduced karyoplasmic ratio and abundant organelles were observed in the co-cultured MSCs by transmission electron microscopy, and cell fusion can be seen.

    Conclusions UVECs induce MSCs to differentiate into endothelial cells through direct cell contact by direct co-culture, the mechanism may be closely related to the formation of gap junction and the promotion of VEGF secretion by in direct contact and cell fusion.

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